Hormonal control of reproduction and growth in fish

鱼类繁殖和生长的激素控制

• 批准号: 156910-2012
• 负责人: Habibi, Hamid
• 金额: $ 2.62万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=522704
• 关键词: Hormonal; control; reproduction; growth; fish

My long-term objective over many years has been to elucidate hormonal control of reproduction and growth in fish. The focus here is to investigate the control of reproduction and growth by a number of key factors, including gonadotropin releasing hormone (GnRH), gonadal steroids, gonadotropin-inhibitory hormone (GnIH) that belongs to RFamide-related family of peptides, and thyroid hormones. Recent findings in my laboratory provided critical information on estrogen and thyroid receptor subtypes structure and function in goldfish. Our experimental results also provide important information concerning seasonal effects of GnIH on pituitary gonadotropin (GTH) and growth hormone (GH) production, and the role of thyroid hormones in the regulation of reproduction. The proposed research will continue the work in progress and initiate new lines of inquiry concerning integrated control of pituitary GTH and GH production and gonadal development, using fish as suitable experimental model. The main hypothesis to be tested is that hypothalamic neurohormones, GnRH and GnIH by controlling pituitary LH, FSH & GH production (Objective 1) work in an integrated manner with thyroid hormones (Objective 2) to control gonadotropic and somatotropic axis, and regulate growth development and differentiation. The proposed study will use a system approach, using advanced transcriptome and metabolomic approach to understand complex biological processes that regulate reproduction and growth. The proposed study will provide novel information on the mechanisms of action of GnIH, GnRH and thyroid hormones, using goldfish and zebrafish as experimental model. The proposed research will provide a suitable vehicle for high quality training of students in the areas of molecular comparative endocrinology. The multi-disciplinary research in my lab assures that students are exposed to an intellectually stimulating environment, a wide range of techniques, and a suitable learning environment in their own area of research as well as outside their narrow areas of specializations.

多年来，我的长期目标一直是阐明鱼类生殖和生长的激素控制。本研究的重点是探讨生殖和生长的一些关键因素的控制，包括促性腺激素释放激素（GnRH），性腺类固醇，促性腺激素抑制激素（GnIH），属于RFamide相关的肽家族，和甲状腺激素。最近在我的实验室的研究结果提供了关键信息的雌激素和甲状腺受体亚型的结构和功能的金鱼。我们的实验结果还提供了重要的信息，季节性影响的GnIH对垂体促性腺激素（GTH）和生长激素（GH）的生产，以及甲状腺激素在生殖调节的作用。拟议的研究将继续进行中的工作，并启动新的调查线有关综合控制垂体GTH和GH的生产和性腺发育，使用鱼作为合适的实验模型。下丘脑神经激素GnRH和GnIH通过控制垂体LH、FSH和GH的产生（目的1），与甲状腺激素（目的2）共同控制促性腺激素和促生长激素轴，调节生长发育和分化。拟议的研究将使用系统方法，使用先进的转录组学和代谢组学方法来理解调节生殖和生长的复杂生物过程。这项研究将提供新的信息，促性腺激素释放激素，促性腺激素释放激素和甲状腺激素的作用机制，使用金鱼和斑马鱼作为实验模型。拟议的研究将提供一个合适的车辆，高质量的培养学生在分子比较内分泌学领域。我实验室的多学科研究确保学生在自己的研究领域以及他们狭窄的专业领域之外接触到智力刺激的环境，广泛的技术和合适的学习环境。