Hormonal control of reproduction and growth in fish

鱼类繁殖和生长的激素控制

• 批准号: 156910-2012
• 负责人: Habibi, Hamid
• 金额: $ 2.62万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=545754
• 关键词: Hormonal; control; reproduction; growth; fish

My long-term objective over many years has been to elucidate hormonal control of reproduction and growth in fish. The focus here is to investigate the control of reproduction and growth by a number of key factors, including gonadotropin releasing hormone (GnRH), gonadal steroids, gonadotropin-inhibitory hormone (GnIH) that belongs to RFamide-related family of peptides, and thyroid hormones. Recent findings in my laboratory provided critical information on estrogen and thyroid receptor subtypes structure and function in goldfish. Our experimental results also provide important information concerning seasonal effects of GnIH on pituitary gonadotropin (GTH) and growth hormone (GH) production, and the role of thyroid hormones in the regulation of reproduction. The proposed research will continue the work in progress and initiate new lines of inquiry concerning integrated control of pituitary GTH and GH production and gonadal development, using fish as suitable experimental model. The main hypothesis to be tested is that hypothalamic neurohormones, GnRH and GnIH by controlling pituitary LH, FSH & GH production (Objective 1) work in an integrated manner with thyroid hormones (Objective 2) to control gonadotropic and somatotropic axis, and regulate growth development and differentiation. The proposed study will use a system approach, using advanced transcriptome and metabolomic approach to understand complex biological processes that regulate reproduction and growth. The proposed study will provide novel information on the mechanisms of action of GnIH, GnRH and thyroid hormones, using goldfish and zebrafish as experimental model. The proposed research will provide a suitable vehicle for high quality training of students in the areas of molecular comparative endocrinology. The multi-disciplinary research in my lab assures that students are exposed to an intellectually stimulating environment, a wide range of techniques, and a suitable learning environment in their own area of research as well as outside their narrow areas of specializations.

多年来我的长期目标是阐明激素对鱼类繁殖和生长的控制。这里的重点是研究一些关键因素对生殖和生长的控制，包括促性腺激素释放激素 (GnRH)、性腺类固醇、属于 RFamide 相关肽家族的促性腺激素抑制激素 (GnIH) 和甲状腺激素。我实验室的最新研究结果提供了有关金鱼雌激素和甲状腺受体亚型结构和功能的重要信息。我们的实验结果还提供了有关 GnIH 对垂体促性腺激素 (GTH) 和生长激素 (GH) 产生的季节性影响以及甲状腺激素在生殖调节中的作用的重要信息。拟议的研究将继续正在进行的工作，并以鱼类作为合适的实验模型，启动有关垂体 GTH 和 GH 产生以及性腺发育的综合控制的新研究方向。待检验的主要假设是下丘脑神经激素、GnRH 和 GnIH 通过控制垂体 LH、FSH 和 GH 的产生（目标 1）与甲状腺激素（目标 2）以综合方式发挥作用，控制促性腺轴和生长轴，并调节生长发育和分化。拟议的研究将采用系统方法，利用先进的转录组和代谢组学方法来了解调节繁殖和生长的复杂生物过程。拟议的研究将以金鱼和斑马鱼为实验模型，提供有关 GnIH、GnRH 和甲状腺激素作用机制的新信息。拟议的研究将为分子比较内分泌学领域的学生高质量培训提供合适的工具。我实验室的多学科研究确保学生在自己的研究领域以及狭窄的专业领域之外接触到智力刺激的环境、广泛的技术和合适的学习环境。