Multimode determination of gene transcription and metabolic labeling

基因转录和代谢标记的多模式测定

• 批准号: RTI-2020-00799
• 负责人: Tremblay, André
• 金额: $ 5.11万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691586
• 关键词: Multimode; determination; gene; transcription; metabolic

Nuclear receptors are part of a family of ligand activated proteins that reside in the nucleus of cells and control the expression of specific genes involved in diverse aspects of metabolic functions. To achieve such control, nuclear receptors are directly involved in making contact with target DNA in order to activate or repress specific sets of genes in cells. As such, investigation of how key genes controlling various steps of metabolic pathways are regulated through nuclear receptor activations will greatly improve our understanding of gene transcription and dynamics.***In the laboratory, we have been studying in particular the two estrogen receptors (ERa, ERb) and the PPAR family members based on their respective potential to modulate response to hormones and to control energy metabolism. The proposed equipment is essential to investigate the functional aspects and specific modifications of nuclear receptors in the context of gene transcription, complex assembly, mitochondrial activity and cell phenotypes. The equipment will also allow specific metabolic measurements in respect to other research projects undertaken by the co-applicants. Therefore, several cell biology approaches will be supported in several laboratories to address the role and impact of key effectors and conditions in every research project. The formation and training of highly qualified personnel will also be favorized with the use of the proposed equipment.***Our long term objective is to improve our understanding of how upcoming signals impact cellular processes, in order to provide novel insights in the control of gene network and metabolic outcomes.

核受体是一类配体激活蛋白家族的一部分，它们位于细胞的细胞核中，控制涉及代谢功能不同方面的特定基因的表达。为了实现这种控制，核受体直接参与与目标DNA的接触，以激活或抑制细胞中的特定基因集。因此，研究控制代谢途径各个步骤的关键基因是如何通过核受体的激活来调控的，将极大地提高我们对基因转录和动力学的理解。*在实验室中，我们一直在研究两个雌激素受体(ERA，Erb)和PPAR家族成员，根据它们各自调节激素反应和控制能量代谢的潜力。该设备对于研究核受体在基因转录、复合体组装、线粒体活性和细胞表型等方面的功能和特异性修饰是必不可少的。该设备还将允许对共同申请者进行的其他研究项目进行特定的新陈代谢测量。因此，几个实验室将支持几种细胞生物学方法，以解决关键影响因素和条件在每个研究项目中的作用和影响。高素质人才的形成和培训也将有利于拟议设备的使用。*我们的长期目标是提高我们对即将到来的信号如何影响细胞过程的理解，以便为控制基因网络和代谢结果提供新的见解。