The role of a novel SUMO conjugation process on estrogen receptor function and gene transcription

新型 SUMO 缀合过程对雌激素受体功能和基因转录的作用

• 批准号: 311761-2013
• 负责人: Tremblay, André
• 金额: $ 2.62万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629137
• 关键词: role; novel; SUMO; conjugation; process

Recently, the identification of SUMOylation, the process by which a small modifier protein called SUMO is attached to target proteins, has emerged as a novel mechanism that regulates gene expression. However, the functional consequences of SUMOylation are not very well understood and our goal is to better understand its role in terms of hormone receptors response.Our laboratory has been studying gene expression in response to hormone estrogen and how it interacts with estrogen receptors to mediate its physiological effects at the gene level. In that respect, we have found that estrogen receptors can undergo extensive protein modifications, allowing them to respond to various cellular pathways.We recently showed that one of these modifications was SUMOylation, raising novel aspects in estrogen mode of action. This study aims at characterizing how receptor SUMOylation is important in hormone response and other cellular pathways linked to gene expression. These studies will improve our understanding and bring new opportunities on the role of protein modifications on transcriptional competence and cellular functions.

最近，SUMO化的鉴定，即一种名为SUMO的小修饰蛋白连接到靶蛋白上的过程，已经成为一种调节基因表达的新机制。然而，SUMO化的功能后果还不是很清楚，我们的目标是更好地了解它在激素受体反应中的作用。我们的实验室一直在研究雌激素反应的基因表达，以及它如何与雌激素受体相互作用，在基因水平上调节其生理效应。在这方面，我们发现雌激素受体可以进行广泛的蛋白质修饰，允许它们对不同的细胞途径做出反应。我们最近发现，这些修饰之一是SUMO化，提出了雌激素作用模式的新方面。这项研究的目的是描述受体SUMO化如何在激素反应和其他与基因表达相关的细胞途径中发挥重要作用。这些研究将加深我们对蛋白质修饰在转录能力和细胞功能上的作用的理解，并带来新的机遇。