High Performance Liquid Chromatography for Organic Chemistry

有机化学高效液相色谱

• 批准号: RTI-2020-00018
• 负责人: Back, Thomas
• 金额: $ 5.59万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691997
• 关键词: Performance; Liquid; Chromatography; Organic; Chemistry

***We are requesting funds to replace a 26-year old Waters 600 High Performance Liquid Chromatography (HPLC) instrument that is obsolete and no longer capable of producing usable data. The HPLC is required for almost all of our work and, when functioning, has been used on a daily basis. There is no other instrument available to us that would meet our frequent and often extended usage needs. Our current projects that require this instrument include the discovery of novel classes of selenium-based biological antioxidants that mimic the function of the enzyme GPx. These compounds suppress oxidative stress, which plays a key role in many diseases and degenerative conditions, including heart attacks, strokes and dementia. A related project is the invention of selenium-based catalysts for enantioselective oxidations of synthetic utility. In the area of medicinal chemistry, we are targeting new compounds designed to function as ryanodine receptor inhibitors for potential treatment of heart failure and cardiac arrhythmias, as well as first-in-class nucleoside antimetabolites that target protozoan pathogens such as those that cause malaria and leishmaniasis. Heart failure is a leading cause of death in Canada and other developed countries, while malaria and other protozoan infections affect several hundred million people, mainly in the third world. The HPLC is needed for kinetic measurements used in determining catalytic mechanisms, monitoring and optimizing synthetic reactions, measuring enantiomeric excesses in asymmetric reactions, verifying the purity of products intended for bioassay and for the purification of small samples of highly polar nucleosides, alkaloids and other products that are difficult to purify by other methods. The instrument is also required for the training of HQP at all levels. HPLC is one of the most widely used techniques in organic chemistry laboratories and proficiency in its use is expected of graduating students who wish to work in academic or industrial laboratories such as in the pharmaceutical, biotech or fine chemical areas. I currently supervise 3 Ph.D. students, 1 M.Sc. student and typically 2-3 undergraduates.

***我们请求资金更换一台26年的Waters 600高效液相色谱（HPLC）仪器，该仪器已经过时，不再能够产生可用的数据。我们几乎所有的工作都需要高效液相色谱，并且在运作时，已经在日常工作中使用。我们没有其他工具可以满足我们经常和经常延长使用的需要。我们目前需要该仪器的项目包括发现新型基于硒的生物抗氧化剂，这些抗氧化剂模仿GPx酶的功能。这些化合物抑制氧化应激，氧化应激在许多疾病和退行性疾病中起着关键作用，包括心脏病发作、中风和痴呆。一个相关项目是硒基催化剂的发明，用于对映选择性氧化的合成用途。在药物化学领域，我们的目标是设计用于治疗心力衰竭和心律失常的ryanodine受体抑制剂的新化合物，以及针对原生动物病原体（如引起疟疾和利什曼病的病原体）的一流核苷抗代谢产物。心力衰竭是加拿大和其他发达国家的一个主要死亡原因，而疟疾和其他原生动物感染影响了数亿人，主要是在第三世界。HPLC用于动力学测量，用于确定催化机制，监测和优化合成反应，测量不对称反应中的对映体过量，验证用于生物测定的产品的纯度，以及纯化小样本的高极性核苷，生物碱和其他难以用其他方法纯化的产品。该仪器也需要用于各级HQP的培训。高效液相色谱法是有机化学实验室中使用最广泛的技术之一，希望在制药、生物技术或精细化学领域的学术或工业实验室工作的毕业生能够熟练使用高效液相色谱法。我目前指导3名博士生，1名硕士生和2-3名本科生。