High Performance Liquid Chromatography for Organic Chemistry

有机化学高效液相色谱

• 批准号: RTI-2020-00018
• 负责人: Back, Thomas
• 金额: $ 5.59万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691997
• 关键词: Performance; Liquid; Chromatography; Organic; Chemistry

***We are requesting funds to replace a 26-year old Waters 600 High Performance Liquid Chromatography (HPLC) instrument that is obsolete and no longer capable of producing usable data. The HPLC is required for almost all of our work and, when functioning, has been used on a daily basis. There is no other instrument available to us that would meet our frequent and often extended usage needs. Our current projects that require this instrument include the discovery of novel classes of selenium-based biological antioxidants that mimic the function of the enzyme GPx. These compounds suppress oxidative stress, which plays a key role in many diseases and degenerative conditions, including heart attacks, strokes and dementia. A related project is the invention of selenium-based catalysts for enantioselective oxidations of synthetic utility. In the area of medicinal chemistry, we are targeting new compounds designed to function as ryanodine receptor inhibitors for potential treatment of heart failure and cardiac arrhythmias, as well as first-in-class nucleoside antimetabolites that target protozoan pathogens such as those that cause malaria and leishmaniasis. Heart failure is a leading cause of death in Canada and other developed countries, while malaria and other protozoan infections affect several hundred million people, mainly in the third world. The HPLC is needed for kinetic measurements used in determining catalytic mechanisms, monitoring and optimizing synthetic reactions, measuring enantiomeric excesses in asymmetric reactions, verifying the purity of products intended for bioassay and for the purification of small samples of highly polar nucleosides, alkaloids and other products that are difficult to purify by other methods. The instrument is also required for the training of HQP at all levels. HPLC is one of the most widely used techniques in organic chemistry laboratories and proficiency in its use is expected of graduating students who wish to work in academic or industrial laboratories such as in the pharmaceutical, biotech or fine chemical areas. I currently supervise 3 Ph.D. students, 1 M.Sc. student and typically 2-3 undergraduates.

***我们要求资金代替已过时的，不再能够生成可用数据的2600年水域600高性能液相色谱（HPLC）仪器。几乎所有工作都需要HPLC，并且在运作时每天都使用。我们没有其他可用的工具可以满足我们频繁且经常扩展的使用需求。我们当前需要该仪器的项目包括发现模仿酶GPX功能的新型基于硒的生物抗氧化剂。这些化合物抑制氧化应激，这在许多疾病和退化性疾病中起着关键作用，包括心脏病发作，中风和痴呆症。一个相关的项目是基于硒的催化剂，用于合成效用的对映选择性氧化。在药物化学区域，我们靶向设计的新化合物，旨在用作瑞氨烷受体抑制剂，以治疗心力衰竭和心律不齐的潜在治疗，以及靶向原生动物病原体的第一类核苷抗代谢剂，例如造成疟疾和利什曼病的那些。心力衰竭是加拿大和其他发达国家的主要死亡原因，而疟疾和其他原生动物感染影响了数亿人，主要在第三世界。 HPLC是用于确定催化机制，监测和优化合成反应的动力学测量所需的，测量不对称反应中的对照组过量，验证了针对生物测定的产品的纯度，以及用于纯化高度极性核苷，烷基类产品的小样品和其他产品的纯化。在所有级别上训练HQP也需要该仪器。 HPLC是有机化学实验室中使用的最广泛使用的技术之一，并且在其使用方面的熟练程度是希望毕业的学生希望从事学术或工业实验室的工作，例如在药品，生物技术或精美的化学领域。我目前监督3博士学位学生，1 M.Sc.学生，通常是2-3名本科生。