A high performance liquid chromatography (HPLC) instrument and associated rotary evaporator for small molecule and natural product analyses

用于小分子和天然产物分析的高效液相色谱 (HPLC) 仪器和相关旋转蒸发仪

• 批准号: 389698-2010
• 负责人: Bignell, Dawn
• 金额: $ 7.16万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=442989
• 关键词: performance; liquid; chromatography; HPLC; instrument

This grant will be used to purchase two pieces of equipment: (1) a rotary evaporator, and (2) a High Performance Liquid Chromatography (HPLC) instrument. These instruments will be critical components of a research program focusing on plant pathogenic Streptomyces bacteria that cause potato common scab, which is an economically-important plant disease in Canada. Potato scab is characterized by raised corky-like lesions that form on the surface of potato tubers. These lesions affect the marketability of the potato crop leading to significant economic losses for potato growers. The equipment funded by this grant will be used to identify, quantify and purify natural products that are produced by the best characterized scab-causing pathogen, Streptomyces scabies, and which contribute to the pathogenic character of this organism. The goal of this research is to better understand how these natural products are produced by S. scabies, how they function in disease development, and the environmental conditions that promote the synthesis of the molecules. The results of this research will shed further light on the importance of S. scabies natural products in scab disease development, which has applications for the development of more effective scab management strategies. Another research program that will benefit from the use of this equipment is one that focuses on microbial antibiotic production and new drug discovery. This research program aims to better understand how antibiotics are produced by soil microorganisms so that strategies can be designed to enhance their production for commercial purposes. Furthermore, it aims to characterize new microbial natural products that may be useful as drugs for the treatment of human diseases. The equipment requested will also be important for two additional research programs, one of which studies the involvement of small signaling molecules in virus-mediated genetic exchange in bacteria, and the other which characterizes plant responses to stress. Thus, the instruments funded by this grant will support research programs that have applications to agriculture, biotechnology and medicine, and which also contribute to the advancement of scientific knowledge.

这笔赠款将用于购买两件设备：（1）旋转蒸发器，（2）高效液相色谱仪（HPLC）。这些仪器将是一个研究项目的关键组成部分，该项目的重点是导致马铃薯普通疮痂病的植物病原链霉菌，这是加拿大一种经济上重要的植物疾病。马铃薯疮痂病的特征在于在马铃薯块茎表面上形成凸起的软木样病变。这些病变影响马铃薯作物的适销性，导致马铃薯种植者的重大经济损失。这笔赠款资助的设备将用于鉴定、量化和纯化由最具特征的引起疥疮的病原体疥疮链霉菌产生的天然产物，这些天然产物有助于这种生物体的致病特性。本研究的目的是更好地了解这些天然产物是如何产生的S。疥疮，它们如何在疾病发展中发挥作用，以及促进分子合成的环境条件。本研究的结果将进一步阐明S.疥疮天然产物在疮痂病发展中的作用，可用于制定更有效的疮痂管理策略。另一个将从使用这种设备中受益的研究项目是专注于微生物抗生素生产和新药发现的项目。该研究计划旨在更好地了解土壤微生物如何产生抗生素，以便可以设计策略来提高其商业用途的生产。此外，它的目的是表征可能用作治疗人类疾病的药物的新的微生物天然产物。所要求的设备对另外两个研究项目也很重要，其中一个项目研究小信号分子参与病毒介导的细菌遗传交换，另一个项目研究植物对胁迫的反应。因此，这笔赠款资助的仪器将支持应用于农业，生物技术和医学的研究计划，并有助于科学知识的进步。