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MethylTrap : chemical affinity reagents for reliable and reproducible PTM-targeted proteomics - Phase 1 application

MmethylTrap：用于可靠且可重复的 PTM 靶向蛋白质组学的化学亲和试剂 - 第一阶段应用

基本信息

批准号：
544479-2019
负责人：
Hof, Fraser
金额：
$ 9.11万
依托单位：
University of Victoria
依托单位国家：
加拿大
项目类别：
Idea to Innovation
财政年份：
2019
资助国家：
加拿大
起止时间：
2019-01-01 至 2020-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693385
关键词：
MethylTrap chemical affinity reagents reliable

项目摘要

Methylation is a modification that is added to proteins as a part of natural processes. It is small, but powerful. When methyl groups are added to or removed from proteins, they serve as switches that govern many on/off changes of biological events. It is often important for health and disease. For example, methylation is associated in multiple instances with the change of treatable cancer to a more aggressive form of the disease. There are expected to be 100's or 1000's of methylated proteins in any given biological sample, but researchers can often only identify a small part of them because they are present in very small amounts.We have developed molecules that can selectively bind to portions of methylated proteins, and in doing so can enrich them from complex biological samples. We are aiming to commercialize this methyl enrichment technology. This technology could be used by researchers to discover more methylated proteins in many kinds of samples. In order to prove the value of this technology, we are aiming to demonstrate its ability to enrich samples from multiple kinds of cancer cells, we are aiming to demonstrate that it can be used in quantitative experiments that measure absolute amounts of methylated analytes, and we are aiming to prove that we can enrich and identify methylated proteins from real human biological fluid samples.We aim to make an impact in two distinct markets. This reagent will power new basic science discoveries about methylation, when made available as a research tool. This reagent will also ultimately enable new clinical diagnostics for many diseases that involve methylated proteins markers, when made available to those who specialize in biomarker discovery and clinical testing.

甲基化是作为自然过程的一部分添加到蛋白质中的修饰。它很小，但很强大。当甲基被添加到蛋白质中或从蛋白质中去除时，它们充当控制生物事件的许多开/关变化的开关。它通常对健康和疾病很重要。例如，甲基化在多种情况下与可治疗的癌症向更侵袭性的疾病形式的变化相关。在任何给定的生物样品中，预计会有100或1000的甲基化蛋白质，但研究人员通常只能识别其中的一小部分，因为它们存在的量非常小。我们已经开发出可以选择性地结合部分甲基化蛋白质的分子，这样做可以从复杂的生物样品中富集它们。我们的目标是将这种甲基富集技术商业化。这项技术可以被研究人员用来发现更多的甲基化蛋白质在许多种类的样品。为了证明这项技术的价值，我们的目标是证明它能够从多种癌细胞中富集样品，我们的目标是证明它可以用于定量实验，测量甲基化分析物的绝对量，我们的目标是证明我们可以从真实的人类生物体液样本中富集和鉴定甲基化蛋白质。我们的目标是在两个不同的市场产生影响。这种试剂将推动关于甲基化的新的基础科学发现，当作为一种研究工具可用时。该试剂还将最终为许多涉及甲基化蛋白质标记物的疾病提供新的临床诊断方法，当这些试剂可用于生物标记物发现和临床测试的专业人员时。