Magnetic Tweezer Force Spectroscopy for Mechanotransduction Studies

要做的工作的性质：心血管疾病，如冠状动脉闭塞，是加拿大的主要死亡原因之一。对于有严重冠状动脉阻塞的患者，经常使用基于导管的干预措施，如冠状动脉成形术和支架置入术。然而，血管成形术和支架置入术受到不受欢迎的和不受控制的平滑肌细胞增殖的限制，这些细胞最终会重新闭塞动脉。通过正在进行的NSERC DG和DAS资金，该研究计划将专注于开发新的使能技术，通过将Jagged1 Notch配体与支架进行功能结合来应对与支架相关的挑战，以期改善血管修复结果。这项RTI资金申请是为了为研究计划购买急需的磁镊力光谱学。*为什么研究对谁重要以及预期的结果：拟议的研究很重要，因为它将生物材料和生物学与心脏病结合在一起。这项拟议的研究将为支架性能中尚未解决的挑战之一提供基本的理解。申请人期望开发具有结合Jagged1的支架，以阻止导致再闭塞的不受欢迎的平滑肌细胞增殖。在这一过程中，还有望获得基础材料诱导的细胞生物学知识。*对研究领域和加拿大的好处：拟议的研究利用一种类型的生物材料(支架)将Jagged1呈现给细胞。拟议的研究将通过开拓医疗支架和基于生物材料的治疗技术部门的新市场，为未来的人类健康和经济利益带来重大的技术开发。**

The nature of the work to be done: Cardiovascular disease such as coronary artery occlusion is one of the leading causes of death in Canada. For patients with severe coronary artery blockage, catheter-based interventions such as coronary angioplasty and stenting are frequently utilized. However, angioplasty and stenting are limited by the undesired and uncontrolled proliferation of smooth muscle cells that eventually reocclude the artery. Through the ongoing NSERC DG and DAS funding, the research program will focus on developing novel enabling technologies to address the challenges associated with stenting by functional conjugation of Jagged1 Notch ligand to stents with the aim of improving vascular reparative outcome. This RTI funding request is to purchase a critically needed magnetic tweezer force spectroscopy for the research program.******Why and to whom the research is important and the anticipated outcomes: The proposed research is important because it brings together biomaterials and biology to heart disease. The proposed research will provide fundamental understandings about one of the unsolved challenges in stent performance. The applicant anticipates to develop stents with conjugated Jagged1 that arrests the undesired smooth muscle cell proliferation that leads to reocclusion. In the process, gaining fundamental materials-induced cell biology knowledge is also anticipated. ******The benefits to the research field and to Canada: The proposed research utilizes one type of biomaterials (stents) to present Jagged1 to cells. The proposed research will result in significant technology development for applications in future human health and economic benefit to Canada by opening up new markets in the medical stent and biomaterials-based therapeutic technology sector.**