The isothermal amplification technology as an innovative biological tool for modern agriculture

从土壤中驯化良好的细菌和真菌，并在现代农业中引入和实施，对于减少与土地利用增加有关的环境破坏至关重要。当土地被集约用于农业生产时，土壤肥力迅速下降。当农用化学品被用来缓解养分流失和提高作物年产量时，除了经济损失外，它还一再导致严重的环境问题。在农业中使用有益微生物可以保持土壤健康，确保可持续利用不可再生资源，此外还有助于提高作物质量和产量，同时大大降低每公顷农用化学品的相关成本。因此，在农业中引入有益微生物是至关重要的。我们最近观察到这些微生物在批量生产的质量和确定这些生物制品的正确保质期方面的技术进步。尽管取得了这些进展，但生物肥料生产中最关键的问题之一是缺乏适当的工具来在田间准确和直接地追踪这些生物。确实需要开发可靠和快速的方法来量化这些引入的有益微生物物种。在这方面，利用重组酶聚合酶扩增(RPA)的等温扩增技术是一种强大的分子技术，当直接用于现场诊断时已被证明是成功的。该方法具有价格实惠、灵敏度高、反应时间短、稳定性好、操作简便等优点，得到了广泛的应用。这一行业主导的项目旨在开发一种低成本、基于RPA的半定量方法，用于快速可靠地检测环境样品中的有益微生物。这项技术将满足现场对可靠的微生物诊断服务日益增长的市场需求。

The domestication of good bacteria and fungi from soil and their introduction and implementation in modern agriculture is of paramount importance for reducing environmental damage linked to land-use increase. When land is used intensively for agricultural production, soil fertility declines rapidly. When agro-chemicals are used to mitigate the nutrient loss and increase annual crop yields, it repeatedly leads to serious environmental problems in addition to economic losses. The use of beneficial microorganisms in agriculture can preserve soil health and ensures sustainable use of non-renewable resources in addition to help increase the crop quality and yield while greatly reducing agro-chemicals associated cost per hectare. Hence, the introduction of beneficial microorganisms in agriculture is of critical importance. We recently observed technological improvements in the quality of those microorganisms mass-production and in the determination of a correct shelf life for those biological products. Despite these advances, one of the most critical points in biological fertilizer production is the lack of appropriate tools for precise and direct tracing of these organisms in the field. There is a real need to develop reliable and fast methods for quantifying these introduced beneficial microbial species. In that regard, the isothermal amplification technology using recombinase polymerase amplification (RPA) is a powerful molecular technique that has proven successful when used directly in the field for diagnosis. The method has been widely used due to its affordable price, high sensitivity, short reaction times, robustness and simplicity. This industry-driven project aims to develop a low-cost, semi quantitative RPA-based method for fast and reliable detection of beneficial microorganisms in environmental samples. This technique will meet the increased market demand for reliable microbial diagnosis services in the field.