The isothermal amplification technology as an innovative biological tool for modern agriculture

等温扩增技术作为现代农业的创新生物工具

• 批准号: 537126-2018
• 负责人: Méthot, Nathalie
• 金额: $ 5.19万
• 依托单位: La Cité collégiale
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=698942
• 关键词: isothermal; amplification; technology; innovative; biological

The domestication of good bacteria and fungi from soil and their introduction and implementation in modern agriculture is of paramount importance for reducing environmental damage linked to land-use increase. When land is used intensively for agricultural production, soil fertility declines rapidly. When agro-chemicals are used to mitigate the nutrient loss and increase annual crop yields, it repeatedly leads to serious environmental problems in addition to economic losses. The use of beneficial microorganisms in agriculture can preserve soil health and ensures sustainable use of non-renewable resources in addition to help increase the crop quality and yield while greatly reducing agro-chemicals associated cost per hectare. Hence, the introduction of beneficial microorganisms in agriculture is of critical importance. We recently observed technological improvements in the quality of those microorganisms mass-production and in the determination of a correct shelf life for those biological products. Despite these advances, one of the most critical points in biological fertilizer production is the lack of appropriate tools for precise and direct tracing of these organisms in the field. There is a real need to develop reliable and fast methods for quantifying these introduced beneficial microbial species. In that regard, the isothermal amplification technology using recombinase polymerase amplification (RPA) is a powerful molecular technique that has proven successful when used directly in the field for diagnosis. The method has been widely used due to its affordable price, high sensitivity, short reaction times, robustness and simplicity. This industry-driven project aims to develop a low-cost, semi quantitative RPA-based method for fast and reliable detection of beneficial microorganisms in environmental samples. This technique will meet the increased market demand for reliable microbial diagnosis services in the field.

从土壤中驯化优良细菌和真菌，并将其引入现代农业，对于减少与土地使用增加有关的环境损害至关重要。当土地被集中用于农业生产时，土壤肥力迅速下降。当使用农用化学品来减少养分流失和增加作物年产量时，除了经济损失外，还一再导致严重的环境问题。在农业中使用有益微生物可以保持土壤健康，确保可持续利用不可再生资源，此外还有助于提高作物质量和产量，同时大大降低每公顷农用化学品的相关成本。因此，在农业中引入有益微生物至关重要。 我们最近观察到这些微生物大规模生产的质量和确定这些生物产品的正确保质期方面的技术改进。尽管取得了这些进展，但生物肥料生产中最关键的一点是缺乏适当的工具来精确和直接追踪田间的这些生物。真实的需要开发可靠和快速的方法来量化这些引入的有益微生物物种。在这方面，使用重组酶聚合酶扩增（RPA）的等温扩增技术是一种强大的分子技术，其在直接用于诊断领域时已被证明是成功的。该方法因其价格合理、灵敏度高、反应时间短、稳定性好、操作简便而得到广泛应用。该行业驱动的项目旨在开发一种低成本，半定量的基于RPA的方法，用于快速可靠地检测环境样品中的有益微生物。该技术将满足市场对可靠的微生物诊断服务的需求。