Towards a better understanding of contaminant bioavailability- urgent replacement of photosynthetic incubators

更好地了解污染物的生物利用度——紧急更换光合培养箱

• 批准号: RTI-2022-00353
• 负责人: Wilkinson, Kevin
• 金额: $ 9.03万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743060
• 关键词: Towards; better; understanding; contaminant; bioavailability

This is an urgent application to replace an incubator system that will be used mainly to relate the chemical speciation of contaminants to their biological availability. It consists of three autonomous, stacked photosynthetic incubators with temperature (ambient-20 to 60 oC), lighting and mixing controls. The incubators are used to produce microbial cultures (both bacterial and algal) and grow plant seedlings that are subsequently exposed to a variety of microcontaminants under varying physico-chemical conditions. The microorganisms are typically analyzed for their biouptake and for biological effects based upon a number of (transcriptomic) biomarkers. For example, the incubators have recently been used to study the biouptake of rare earth metals; to identify transcriptomic biomarkers for Ce nanoparticles; to determine the fate of Ag nanoparticles or to develop multiplexed surface-enhanced Raman spectroscopy (SERS) aptasensors to detect microcystins in surface waters. Since most of the work in our lab examines interactions between contaminants and microorganisms, the incubators are critical for the reproducible production of cultures, which are used to systematically evaluate chemical bioavailability. The incubators that we are currently using were acquired while I was working at the University of Geneva (purchased in 2002) and transported to the University of Montreal in 2005. They have been repaired numerous times, however, only a single incubator (of the three) is currently functional. Given the nature of our research, the incubators are a critical component of the training of most of the HQP in my diverse research group and within collaborative research projects. They will contribute to a modern training environment in multidisciplinary environmental sciences that will position the students for future meaningful employment.

这是一个迫切的应用程序，以替换将主要用于将污染物的化学物种形成与其生物学可用性联系起来的孵化器系统。它由三个具有温度（环境20至60 oC）的自动堆叠的光合孵化器组成，照明和混合控件。孵化器用于产生微生物培养物（细菌和藻类），并种植植物幼苗，随后在不同的物理化学条件下暴露于各种微抑制剂。通常对微生物进行生物蛋糕和基于许多（转录）生物标志物的生物学作用进行分析。例如，孵化器最近被用来研究稀土金属的生物蛋糕。鉴定CE纳米颗粒的转录组生物标志物；确定Ag纳米颗粒的命运或发展多发性表面增强的拉曼光谱法（SERS）Aptasensors以检测地表水中的微囊蛋白。由于我们实验室中的大多数工作都检查了污染物和微生物之间的相互作用，因此孵化器对于可再现的培养物生产至关重要，这些培养物用于系统地评估化学生物利用度。我们目前正在使用的孵化器是在我在日内瓦大学工作（2002年购买）并于2005年运送到蒙特利尔大学时获得的。但是，它们已经进行了多次维修，但是，目前只有一个（三个）的单个孵化器才能使用。鉴于我们的研究性质，孵化器是我多元化研究小组和协作研究项目中大多数HQP培训的关键组成部分。他们将为多学科环境科学的现代培训环境做出贡献，这些培训将使学生为未来的有意义的就业定位。