Adaptive optics applied to laser femtosecond filamentation for radiation sciences applications

高强度聚焦超短激光脉冲常用于生物医学研究和治疗。最近，超短脉冲激光不仅用于双光子显微镜，而且还用于通过完整颅骨对小鼠大脑进行高分辨率体内成像。在治疗方面，我们已经展示了基于飞秒红外激光束照射的新的癌症放射治疗（R。Meesat等人PNAS第109卷，E2508-E2513（2012），专利USA 13/580，038（2012），PCT W 02011/109907 Al（2011），专利CA PCT/CA 2011/000273，韩国PCT 5-2012-048051-9（2011））。在放射生物学和放射疗法中，超短激光脉冲在称为双折射的非线性光子过程中产生低能电子（LEE），称为低密度等离子体。这种利用自适应光学方法的激光诱导LEE可用于生物功能调节。为了实现低密度等离子体的空间微观控制，我们将引入可编程空间光调制器（SLM）。自适应光学方法将产生使用SLM，并将被应用于控制的飞秒激光照射的放射生物学和放射治疗的应用。LEE飞秒等离子体沉积的微观控制可以自动调节，并且与特定的超短激光无关。这种激光等离子体微束将用于未来的治疗（放射生物学和放射疗法），成像和更广泛的生物功能调制。 细丝是辐射科学中的轨迹的模拟物，但有一个重要的区别：凝聚态物质（水）中细丝的直径约为5.5米。一般情况下，高斯型光强分布不平滑的激光束会产生多个灯丝的聚束。SLM自适应光学方法将使自调节单丝独立于飞秒激光。剂量等效飞秒能量沉积（飞秒剂量沉积）的真实的时间微观基础是总体的工程目标。我们将测量这种自调节单丝对放射生物学感兴趣的生物分子的影响。第一次，它将有可能成像的组织，选择一个真实的时间干预（可逆的低密度等离子体光调制，或不可逆的低密度等离子体放射治疗）使用相同的飞秒激光系统调制和SLM自适应光学方法。

Focused ultrashort laser pulses with high intensity is often used in biomedical research and treatment. Recently ultrashort-pulsed lasers have been used not only for two-photons microscopy but also in high resolution in vivo imaging of mouse brain through the intact skull. In therapy, we have shown new cancer radiotherapy based on femtosecond IR laser-beam filamentation (R. Meesat et al. PNAS vol. 109, E2508-E2513 (2012), Patent USA 13/580,038 (2012), PCT WO2011/109907 A1 (2011), Patent CA PCT/CA2011/000273, Korean PCT 5-2012-048051-9 (2011)). In radiobiology and radiotherapy ultrashort laser pulses generate low energy electrons (LEE), known as low-density plasma in a nonlinear photonic process called filamentation. This laser-induced LEE using adaptive optics method can be used for biological function modulation. To achieve this spatially microscopic control of the low-density plasma, we will introduce a programmable Spatial Light Modulator (SLM). An adaptive optics method will be generated using the SLM and will be applied to the control of the femtosecond laser filamentation for radiobiology and radiotherapy applications. The microscopic control of the LEE femtosecond plasma deposition can be auto-regulated and independent of the specific ultrashort laser. This laser plasma micro-beam will be used for future treatments (radiobiology and radiotherapy), imaging and more generally modulation of biological functions. Filaments are analogue of tracks in radiation science with an important difference: diameter of filaments in condensed matter (water), are around 5.5 m. In general, the Gaussian profile intensity of the laser beam is not smooth will produced a bunching of multiple filaments. The SLM adaptive optics method will trig a self-regulated monofilament independent of the femtosecond laser. The real time microscopic basis of the dose equivalent femtosecond energy deposition of radiation (femtosecond Dose deposition) is the general project aim. We will measure the effects of this auto-regulated monofilament on biomolecules of radiobiological interest. For the first time, it will be possible to image a tissue, choosing an real time intervention (reversible low-density plasma photomodulation, or irreversible low-density plasma radiotherapy) using the same fs laser system modulated by and SLM adaptive optics method.