Metabolic Analyzer for the study of live cells and tissue explants

用于研究活细胞和组织外植体的代谢分析仪

• 批准号: RTI-2021-00509
• 负责人: Beaudry, Jacqueline
• 金额: $ 10.81万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718322
• 关键词: Metabolic; Analyzer; study; live; cells

In this proposal the funds requested will support an Agilent Seahorse Metabolic Analyzer XFe24 instrument, which uses live cells or tissue explants in a 24-well plate format to measure oxygen consumption, mitochondrial stress and extracellular acidification rates. It allows the study of cell or tissue specific responses to substrates, inhibitors and other peptides like hormones in real-time. The purchase of this instrument will greatly support the research programs of Drs. Beaudry, Comelli, Bazinet, O'Connor, and Anderson, five investigators in the Department of Nutritional Sciences at the University of Toronto and Dr. Sung, an investigator at the SickKids Research Institute. Together these researchers have mutual interests in understanding fundamental mechanisms by which nutrient and environmental factors affect physiology. The above mentioned, all have active NSERC DGs for which the requested equipment will be applied. Dr. Jacqueline Beaudry is a newly appointed (July 2020) Assistant Tenure-track Professor and principle applicant on this proposal. The overall objective of her research program is to understand how fat is influenced by glucocorticoids (GCs) or stress hormones. Her interests are focused on the essential mechanisms involved in GC signaling within the white (used for fat storage) and brown (used for dissipation of energy) fat tissue and how it regulates body weight. This instrument will allow intricate investigation into the function of how GC pathways affect mitochondrial function within fat tissue. This will lead to a better understanding of how chronic and high levels of GCs can increase fat tissue growth, which impacts overall body weight. Importantly, this machine will be used by all HQP supervised under the listed co-applicants, reinstating the desire and need for this instrument as a shared part of the Beaudry laboratory. Dr. Comelli will use the equipment to study explants and cell lines of the intestine and how they interact with the gut microbiota and its metabolites. Dr. Bazinet will investigate fatty acid substrates and how they interact with fat tissue. Dr. O'Connor will study the impact of folic acid and how it affects the availability and absorption of colonic folate. Dr. Anderson will focus his investigations in gut-brain signals involved in food intake and glucose regulation in response to amino and/or fatty acid receptor antagonists or agonists. Dr. Sung will focus his experiments on fat tissue plasticity and will investigate the adaptations to metabolic challenges that induce browning of white fat tissue. Procurement of this equipment will provide the first accessible Seahorse instrument to St. George Campus, at the University of Toronto. It will position Dr. Beaudry and co-applicants' laboratories for success in creating high-level research and impactful HQP training, ensuring the University of Toronto maintains its position as a leading research institute in Canada and remains competitive globally.

在这项提案中，所要求的资金将支持Agilent Seahorse代谢分析仪XFe 24仪器，该仪器使用24孔板格式的活细胞或组织外植体来测量耗氧量，线粒体应激和细胞外酸化率。它允许实时研究细胞或组织对底物，抑制剂和其他肽类激素的特异性反应。 购买这台仪器将极大地支持Beaudry、Comelli、Bazinet、奥康纳和安德森博士的研究计划，他们是多伦多大学营养科学系的五名研究人员，以及SickKids研究所的研究人员Sung博士。这些研究人员在了解营养和环境因素影响生理的基本机制方面有着共同的兴趣。上述所有设备都具有将应用所请求设备的活动NSERC DG。 博士杰奎琳Beaudry是一个新任命的（2020年7月）助理终身教授和主申请人在这个建议。她的研究计划的总体目标是了解糖皮质激素（GC）或应激激素如何影响脂肪。她的兴趣集中在白色（用于脂肪储存）和棕色（用于能量耗散）脂肪组织中GC信号传导的基本机制以及它如何调节体重。该仪器将允许对GC途径如何影响脂肪组织内线粒体功能的功能进行复杂的调查。这将导致更好地了解慢性和高水平的GC如何增加脂肪组织生长，从而影响整体体重。 重要的是，这台机器将由列出的共同申请人监督下的所有HQP使用，恢复了对这台仪器作为Beaudry实验室共享部分的愿望和需求。Comelli博士将使用该设备研究肠道的外植体和细胞系，以及它们如何与肠道微生物群及其代谢产物相互作用。Bazinet博士将研究脂肪酸底物以及它们如何与脂肪组织相互作用。奥康纳博士将研究叶酸的影响，以及它如何影响结肠叶酸的可用性和吸收。安德森博士将集中在肠脑信号参与食物摄入和葡萄糖调节反应氨基和/或脂肪酸受体拮抗剂或激动剂的研究。宋博士将把他的实验重点放在脂肪组织的可塑性，并将调查适应代谢的挑战，诱导白色脂肪组织的布朗宁。 该设备的采购将为多伦多大学圣乔治校区提供第一台可访问的Seahorse仪器。它将使Beaudry博士和共同申请人的实验室成功地创建高水平的研究和有影响力的HQP培训，确保多伦多大学保持其作为加拿大领先研究机构的地位，并保持全球竞争力。