Design of a new PLLA vascular scaffold coated with stem cell-derived nanovesicles for critical limb ischemia

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Vascular scaffolds have been widely studied for the treatment of critical limb ischemia (CLI). However, the risk of postoperative restenosis raises the need of other factors that regulate inflammation or intimal growth. A combination therapy with mechanical support using a bioresorbable scaffold (bioresorbable stent) and biochemical cues from stem cell-derived nanovesicles as a coating for drug deliver is put forward for better patient outcomes and faster recoveries.In this study, the main objective is to design, develop and test a new bioresorbable scaffold coated stent based on stem cell-derived nanovesicles for higher biocompatibility and enhanced therapeutic efficacy. Stent geometries will be generated and modelled numerically for assessing deployment and drug dispersion kinetics. The best candidates will be prototyped with laser micro-machining. In collaboration with the partner, the prototypes will be coated with the nano-seicles technology. Subsequently, the drug dispersion kinetics of the coated prototypes will tested using a dedicated hydraulic setup reproducing the anatomical structures and physiological flow conditions. The partner, will provide the design criteria and verify the design performance with 3D OCT imaging (for scaffold deployment assessment) and assist for the laser prototyping and mechanical performance testing.