Design of a new PLLA vascular scaffold coated with stem cell-derived nanovesicles for critical limb ischemia

设计一种涂有干细胞衍生纳米囊泡的新型 PLLA 血管支架，用于治疗严重肢体缺血

• 批准号: 568480-2021
• 负责人: Mongrain, Rosaire
• 金额: $ 1.46万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=723107
• 关键词: Design; new; PLLA; vascular; scaffold

Vascular scaffolds have been widely studied for the treatment of critical limb ischemia (CLI). However, the risk of postoperative restenosis raises the need of other factors that regulate inflammation or intimal growth. A combination therapy with mechanical support using a bioresorbable scaffold (bioresorbable stent) and biochemical cues from stem cell-derived nanovesicles as a coating for drug deliver is put forward for better patient outcomes and faster recoveries.In this study, the main objective is to design, develop and test a new bioresorbable scaffold coated stent based on stem cell-derived nanovesicles for higher biocompatibility and enhanced therapeutic efficacy. Stent geometries will be generated and modelled numerically for assessing deployment and drug dispersion kinetics. The best candidates will be prototyped with laser micro-machining. In collaboration with the partner, the prototypes will be coated with the nano-seicles technology. Subsequently, the drug dispersion kinetics of the coated prototypes will tested using a dedicated hydraulic setup reproducing the anatomical structures and physiological flow conditions. The partner, will provide the design criteria and verify the design performance with 3D OCT imaging (for scaffold deployment assessment) and assist for the laser prototyping and mechanical performance testing.

血管支架在治疗严重肢体缺血（CLI）方面已得到广泛研究。然而，术后再狭窄的风险增加了对调节炎症或内膜生长的其他因素的需求。本研究的主要目的是设计、开发和测试一种基于干细胞衍生纳米囊泡的生物可吸收支架涂层支架，以提高生物相容性和治疗效果。将生成支架几何形状并进行数值建模，以评估展开和药物分散动力学。最好的候选人将使用激光微加工进行原型制作。在与合作伙伴的合作中，原型将涂上纳米seicles技术。随后，将使用再现解剖结构和生理流动条件的专用液压装置测试涂层原型的药物分散动力学。合作伙伴将提供设计标准，并通过3D OCT成像（用于支架展开评估）验证设计性能，并协助激光原型制作和机械性能测试。