Use of nanoparticles to improve seed germination and seedlings abiotic stress tolerance

露天开采、原地开采和线性扰动特征造成的土地退化和碎片化可能会对环境和社会产生不利影响。土地开垦战略与种子引发技术相结合，有可能在人为干扰后改善北方森林的植物建立和恢复植被的成功。本项目包括三个实验，旨在确定多壁碳纳米管(MWCNTs)对选定的北方乡土树种种子萌发、早期成苗和非生物耐逆诱导的影响。试验I的目的是确定纳米微囊化对种子萌发和早期成苗的影响。本研究将研究多壁碳纳米管的最有效组合和浓度，以及层积时间对五种北方本土树种种子萌发的影响，这些树种包括：樱桃、熊莓、普通蓝莓、矮灌木越橘和红醋栗。实验二的目的是研究种子纳米化对北方乡土树种幼苗非生物(盐碱和干旱)胁迫诱导的潜在影响。我们将评估纳米管对非生物抗逆性的影响，并确定提高秋柳、白杨树和颤杨非生物抗逆性的最适碳纳米管浓度。最后，实验三的目的是研究纳米悬浮叶片应用对颤杨、水牛莓、矮灌木蔓越莓和红豆杉幼苗的抗旱性和植物生长的影响。所采用的方法应该为潜在的开垦策略提供洞察，利用现有的农业实践，在非生物胁迫存在的情况下，限制本地北方森林物种的萌发和建立。这些方法也相对便宜，可以大面积应用，并具有长期补救潜力。这项研究也将为大学和学院的研究人员和学生之间提供一个合作的机会。

Land degradation and fragmentation caused by surface mining, in-situ extraction, and linear disturbance features can have adverse environmental and social impacts. Land reclamation strategies combined with seed priming technology can potentially be used to improve plant establishment and revegetation success of boreal forests following anthropogenic disturbances. This project consists of three experiments aiming to determine the effect of multi-walled carbon nanotubes (MWCNTs) on seed germination, early seedling establishment, and abiotic stress tolerance induction in seedlings of selected native boreal forest species. The objective of Experiment I is to determine the effect of nanopriming on seed germination and early seedling establishment. This will investigate the most effective combinations and concentrations of MWCNTs and the duration of stratification on seed germination of five native boreal species, including chokecherry, bearberry, common blueberry, low-bush cranberry, and red currant. The objective of Experiment II is to investigate the potential effect of seed nanopriming on abiotic (salinity and drought) stress tolerance induction in seedlings of selected native boreal species. We will evaluate the effects of nanopriming on abiotic stress tolerance and determine the optimum concentration of CNTs to improve the abiotic stress tolerance of autumn willow, Bebb's willow, and trembling aspen. Finally, the objective of Experiment III is to investigate the effect of nano-suspension foliar application on drought tolerance and plant growth in seedlings of trembling aspen, buffaloberry, low bush cranberry, and red osier dogwood.The methods employed should provide insight into a potential reclamation strategy, using established agricultural practices, in the presence of abiotic stressors, limiting native boreal forest species germination and establishment. These methods also remain relatively inexpensive, can be applied over a large area, and have long-term remediation potential. This research will also provide a collaborative opportunity between between University and College researchers and students.