Competing ligand assisted aptamers and DNAzymes for nanomaterials

用于纳米材料的竞争性配体辅助适体和脱氧核糖核酸酶

• 批准号: RGPIN-2020-03875
• 负责人: Liu, Juewen
• 金额: $ 4.66万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=739528
• 关键词: Competing; ligand; assisted; aptamers; DNAzymes

Deoxyribonucleic acid (DNA) sequences that can bind to inorganic nanomaterials with high affinity and specificity are useful for biosensor development, biomineralization, and directed assembly of materials. However, strong nonspecific interactions make it difficult to obtain high quality DNA aptamers for surfaces. In addition, we are interested in exploring surfaces for supporting catalysis. The goal of the current proposal is to select DNA sequences (aptamers) that can selectively bind to inorganic surfaces and DNA-based enzymes (DNAzymes) using inorganic nanoparticles as cofactors for catalysis. Different from previous research, we will use ions and competing molecules to modulate the interaction strength so that nonspecific DNA adsorption is weakened to a degree comparable with the base pairing interactions between DNA. Under such conditions, we may obtain DNA sequences that can specifically recognize surface features. We will choose a few inorganic surfaces including iron oxide, graphene oxide and gold to perform aptamer selections using a large random DNA library. In addition, selection of DNAzymes will be performed in the presence of inorganic nanomaterials with the goal to use the surfaces as cofactors. The selected DNA aptamers and DNAzymes will be carefully characterized in terms of binding strength and catalytic efficiency. Their interactions with the target surfaces will be studied using various spectroscopic techniques, and their analytical and materials applications will be explored. This study will broaden our understanding of DNA/nano interfaces, generate specific DNA sequences for surface functionalization and recognition, and produce interesting biosensors and hybrid materials.

脱氧核糖核酸（DNA）序列，可以结合到无机纳米材料具有高亲和力和特异性是有用的生物传感器的发展，生物矿化，和定向组装的材料。然而，强非特异性相互作用使得难以获得用于表面的高质量DNA适体。此外，我们对探索支持催化的表面感兴趣。目前的建议的目标是选择DNA序列（适体），可以选择性地结合到无机表面和基于DNA的酶（DNAzymes）使用无机纳米粒子作为催化的辅因子。与以往的研究不同，我们将使用离子和竞争分子来调节相互作用强度，使非特异性DNA吸附减弱到与DNA之间的碱基配对相互作用相当的程度。在这样的条件下，我们可以获得能够特异性识别表面特征的DNA序列。我们将选择一些无机表面，包括氧化铁、氧化石墨烯和金，使用大型随机DNA库进行适体选择。此外，DNA酶的选择将在无机纳米材料的存在下进行，目的是使用表面作为辅因子。所选择的DNA适体和DNA酶将在结合强度和催化效率方面仔细表征。将使用各种光谱技术研究它们与目标表面的相互作用，并探索它们的分析和材料应用。这项研究将拓宽我们对DNA/纳米界面的理解，产生用于表面功能化和识别的特定DNA序列，并产生有趣的生物传感器和杂化材料。