A high-performance slide scanner to support multiple research programs

支持多个研究项目的高性能载玻片扫描仪

• 批准号: RTI-2022-00514
• 负责人: Martin, Loren
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=741772
• 关键词: performance; slide; scanner; support; multiple

We request funds for a configurable microscope slide scanner system that combines brightfield, fluorescence, and polarization options to provide high-throughput whole slide scanning capabilities to our microscope imaging facility at the University of Toronto Mississauga. A microscope located on campus (in the Martin lab) can scan whole microscope slides; however, this device has a one slide capacity and provides an insufficient workflow. This microscope is also constantly occupied by the Martin lab, making it inaccessible to other researchers. Currently, Dr. Martin's graduate students work in shifts, often past midnight, to have enough time to scan their slides using this microscope. They have expressed serious concerns about their ability to conduct their work, and some have already suffered significant delays. Inefficient workflow and lack of alternative options is not a short-term problem, and Dr. Martin's research will continue to rely centrally on microscope slide scanning. The co-applicants (Liu and Resulaj) also need frequent access to a dedicated slide scanning system, improving their workflow and alleviating the strain on their trainees. In addition, many users at the University of Toronto Mississauga require slide scanning as part of their research program, but our central imaging facility currently lacks a dedicated system. Incorporating a slide scanning system into our microscope imaging facility is expected to improve workflow across multiple investigators and provide a pay-per-use option for investigators (internal and external) not included on the main application. The slide scanning system will be pivotal for the success of our NSERC-funded research programs that aim to understand the molecular and genetic determinants of behaviour. The slide scanning system is necessary to modernize our imaging capabilities, meet our trainees' growing needs, and maximize our research efficiency and originality. The requested equipment will be housed within the Imaging facility at the University of Toronto Mississauga and maintained by a senior research technician. The University of Toronto Mississauga has recently gone through a growth phase with multiple investigators requiring automated slide scanning capabilities for their research programs. An abundance of projects currently depends on microscopy, imaging, and cellular analysis, and it is expected that the proposed equipment will directly support the training of 25-30 students/trainees annually.

我们要求资金用于可配置的显微镜载玻片扫描仪系统，该系统结合了明场、荧光和偏振选项，为多伦多密西索加大学的显微镜成像设施提供高通量的全载玻片扫描能力。位于校园（在马丁实验室）的显微镜可以扫描整个显微镜载玻片;然而，该设备具有一个载玻片的容量，并提供了不足的工作流程。这台显微镜也经常被马丁实验室占用，使其他研究人员无法使用。目前，马丁博士的研究生轮班工作，经常超过午夜，有足够的时间来扫描他们的幻灯片使用这种显微镜。他们对自己开展工作的能力表示严重关切，有些人的工作已经严重拖延。低效的工作流程和缺乏替代方案不是一个短期问题，马丁博士的研究将继续集中依赖于显微镜载玻片扫描。共同申请人（Liu和Resulaj）还需要经常访问专用的幻灯片扫描系统，以改善他们的工作流程并减轻学员的压力。此外，多伦多大学密西索加的许多用户需要将载玻片扫描作为其研究计划的一部分，但我们的中央成像设施目前缺乏专用系统。在我们的显微镜成像设施中安装载玻片扫描系统预计将改善多个研究者的工作流程，并为主应用程序中未包含的研究者（内部和外部）提供按使用付费选项。载玻片扫描系统将是我们NSERC资助的研究项目成功的关键，这些项目旨在了解行为的分子和遗传决定因素。幻灯片扫描系统是必要的，以现代化我们的成像能力，满足我们的学员不断增长的需求，并最大限度地提高我们的研究效率和原创性。 所要求的设备将存放在多伦多大学密西索加的成像设施内，并由一名高级研究技术人员进行维护。多伦多大学密西索加最近经历了一个增长阶段，多个研究人员需要自动幻灯片扫描功能，用于他们的研究计划。目前有大量项目依赖显微镜、成像和细胞分析，预计拟议的设备将直接支持每年培训25-30名学生/受训人员。