Tools for molecular physiology research

分子生理学研究工具

• 批准号: RTI-2022-00118
• 负责人: Goss, Gregory
• 金额: $ 5.93万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743030
• 关键词: Tools; molecular; physiology; research

This RTI application is for a suite of inter-related tools essential for conducting molecular physiology research at Bamfield Marine Sciences Centre, a world-renowned marine research station on the West Coast of Vancouver Island BC. Our collective research is focused on understanding the evolutionary relationships in physiological systems. Our research contributes to the protection and preservation of ecosystems in oceans and seas by focusing on both fundamental molecular physiology and investigation of the effect of human-induced damage to fishes. The nature of this research requires continual access to natural populations of aquatic organisms in these marine environments. The remote nature of our research sites in association with the growing field of genomics necessitates a molecular suite be established at Bamfield Marine Sciences Centre. This will provides the necessary tools to integrate genomics into our molecular physiology programs. We are requesting funds to purchase a molecular suite for research at the Bamfield Marine Sciences Centre in support of our research programs in molecular physiology. This request includes a new liquid scintillation counter necessary for conducting radiotracer-based experiments to understand the cellular and molecular mechanisms of ion transport in marine fishes, a new ultra-low temperature freezer (-80 °C) s required for safe storage and preservation of biological samples, and both a nanodrop spectrophotometer and robust PCR machine that will allow for immediate isolation of RNA and cDNA synthesis for subsequent analysis of gene expression. Each piece of equipment forms part of an interrelated group of equipment that will support multiple NSERC-funded research programs thereby allowing each of the co-applicants plus numerous other NSERC and non-NSERC funded researchers who routinely use BMSC as part of their research to continue advancing research and providing outstanding mentoring of HQP at Bamfield Marine Sciences Centre (BMSC).

此RTI应用程序是一套相互关联的工具，用于在Bamfield海洋科学中心进行分子生理学研究，该中心是位于温哥华岛西海岸的世界知名海洋研究站。我们的集体研究集中在理解生理系统中的进化关系。我们的研究有助于保护和维护海洋生态系统，重点是基础分子生理学和调查人类对鱼类造成的损害的影响。这项研究的性质要求在这些海洋环境中不断获得水生生物的自然种群。我们的研究地点与日益增长的基因组学领域相关联的偏远性质需要在班菲尔德海洋科学中心建立一个分子套件。这将提供必要的工具，将基因组学整合到我们的分子生理学计划。我们正在申请资金购买一套分子套件，用于在班菲尔德海洋科学中心进行研究，以支持我们在分子生理学方面的研究计划。这项请求包括一个新的液体闪烁计数器，用于进行放射性示踪剂实验，以了解海洋鱼类中离子迁移的细胞和分子机制;一个新的超低温冷冻箱（-80 °C），用于安全储存和保存生物样品;以及nanodrop分光光度计和强大的PCR机器，这将允许立即分离RNA和cDNA合成，用于随后的基因分析。表情每台设备都是一组相互关联的设备的一部分，这些设备将支持多个NSERC资助的研究项目，从而允许每个共同申请人以及许多其他NSERC和非NSERC资助的研究人员，他们经常使用BMSC作为他们研究的一部分，继续推进研究，并在班菲尔德海洋科学中心（BMSC）提供HQP的出色指导。