A multicomponent analytical gas chromatography for biological metabolites

气相色谱（GC）广泛用于定量挥发性和半挥发性生物代谢物，并且通常配备有火焰离子化检测器（FID）和质谱仪（MS），其允许以高分辨率准确分析代谢物。此外，当与顶空取样技术结合时，它进一步提高了这种平台的准确性。顶空GC-FID/MS分析非常适用于定量高挥发性和气态分子，包括短链脂肪酸、丙醛和其他含硫化合物，否则在其他色谱平台中难以测量。在这里，我们要求一个多组分气相色谱系统与顶空分析耦合。我们的团队由来自早期，中期和成熟职业的顶尖科学家组成，需要NSERC资助的研究系统继续实现世界级的研究目标。生物化学系没有功能性GC系统，纽芬兰纪念大学的顶空GC系统非常有限。申请人目前将样品送到其他设施，这导致他们的研究严重延误。所要求的设备不仅将加速该团队的研究计划，还将为HQP培训提供新的途径，并提高加拿大在食品和营养研究领域的竞争力。

Gas chromatography (GC) is widely used for quantifying volatile and semi-volatile biological metabolites, and are usually equiped with a flame ionization detector (FID) and mass spectrometer (MS), which allows for accurate analysis of metabolites with high resolution. Moreover, when coupled with headspace sampling technique, it further enhances the accuracy of such platforms. Headspace GC-FID/MS analysis is well-suited for quantifying highly volatile and gaseous molecules, including short chain fatty acids, propanal, and other sulfur-containing compounds, which otherwise are challenging to measure in other chromatographic platforms. Here, we request a multicomponent GC system coupled with headspace analysis. Our team, comprising of top-notch scientists from early, mid, and well-established careers, requires the system for their NSERC-funded research to continue achieving world-class research goals. There is no functional GC system in the Department of Biochemistry and very limited headspace-capable GC system at Memorial University of Newfoundland. The applicants currently send their samples to other facilities, which causes significant delays in their research. The requested equipment will not only accelerates the team's research program but also provide a new avenue for HQP training and increase Canada's competitiveness in the field of food and nutritional research.