A multicomponent analytical gas chromatography for biological metabolites

生物代谢物的多组分分析气相色谱法

• 批准号: RTI-2023-00592
• 负责人: Mayengbam, Shyamchand
• 金额: $ 10.82万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744089
• 关键词: multicomponent; analytical; gas; chromatography; biological

Gas chromatography (GC) is widely used for quantifying volatile and semi-volatile biological metabolites, and are usually equiped with a flame ionization detector (FID) and mass spectrometer (MS), which allows for accurate analysis of metabolites with high resolution. Moreover, when coupled with headspace sampling technique, it further enhances the accuracy of such platforms. Headspace GC-FID/MS analysis is well-suited for quantifying highly volatile and gaseous molecules, including short chain fatty acids, propanal, and other sulfur-containing compounds, which otherwise are challenging to measure in other chromatographic platforms. Here, we request a multicomponent GC system coupled with headspace analysis. Our team, comprising of top-notch scientists from early, mid, and well-established careers, requires the system for their NSERC-funded research to continue achieving world-class research goals. There is no functional GC system in the Department of Biochemistry and very limited headspace-capable GC system at Memorial University of Newfoundland. The applicants currently send their samples to other facilities, which causes significant delays in their research. The requested equipment will not only accelerates the team's research program but also provide a new avenue for HQP training and increase Canada's competitiveness in the field of food and nutritional research.

气相色谱(GC)被广泛应用于挥发性和半挥发性生物代谢物的定量，通常配备有火焰离子化检测器(FID)和质谱仪(MS)，从而能够以高分辨率准确地分析代谢物。此外，与顶空采样技术相结合，进一步提高了此类平台的精度。顶空GC-FID/MS分析非常适合定量高挥发性和气态分子，包括短链脂肪酸、丙醛和其他含硫化合物，否则在其他色谱平台上难以测量。在这里，我们要求多组分GC系统与顶空分析相结合。我们的团队由来自早期、中期和成熟职业生涯的顶尖科学家组成，他们需要NSERC资助的研究系统来继续实现世界级的研究目标。纽芬兰纪念大学的生物化学系没有有效的GC系统，顶空能力非常有限的GC系统。申请者目前将他们的样本发送到其他机构，这导致他们的研究严重延误。所要求的设备不仅将加快团队的研究计划，还将为HQP培训提供新的途径，并增加加拿大在食品和营养研究领域的竞争力。