Sound-Induced 3D Biofabrication and Morphogenesis

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We propose purchasing a unique acoustic bioprinter, CymatiXT (mimiX biotherapeutics, Switzerland, the only vendor of this technology). Sound-induced 3D biofabrication and morphogenesis is a gentle, fast, and easy method to generate multi-cellular, spatially orchestrated tissue constructs using sound waves. This innovative technology provides prospects to overcome some major limitations of conventional methods towards the creation of physiologically relevant in vitro models and engineered tissue constructs. The uniqueness of this technology will push the frontiers of 3D biofabrication both in fundamental and applied research. The requested instrument will uniquely support and complement the mission of our innovative research program to 1) formulate, characterize and validate novel bioinks for 3D bioprinting of human pluripotent stem cell-derived tissues; 2) develop tissue-engineered models using cutting-edge 3D bioprinting and human pluripotent stem cell technologies; 3) transform the drug development process by providing tissue-engineered 3D models capable of faithfully recapitulating the hallmarks of human physiology and disease in vitro. It also supports the vision of our program to produce and validate engineered tissues and organs for replacement and as 3D models for drug safety and efficacy testing. The instrument will be installed at the Research Center of the Sainte Justine Hospital (CRCHUSJ). This will enable all interested users in the CRCHUSJ and University of Montreal to have access to the instrument, which will generate significant interdisciplinary collaborative works. The 3D bioprinter will be used by trainees (HQP) of the applicants and users to acquire hands-on training that will provide them with the marketable skills required for jobs in their disciplines. The 3D bioprinter and team members' laboratories will become attraction poles for highly motivated and qualified students and researchers in biomedical engineering. By acquiring this 3D bioprinter, our innovative program will revolutionize tissue engineering and organ bioprinting and have the spectacular potential for future global leadership in tissue manufacturing. It will also generate social, health, and economic benefits for Canadians, including enhanced training and improved skills for highly qualified personnel. The benefits of this program will be realized by technology transfer activities, intellectual property licensing, creation of new start-up companies, and collaboration with our partners, including the National Research Council of Canada, Montreal TransMedTech Institute, Axelys, and MEDTEQ+, among others.