Sound-Induced 3D Biofabrication and Morphogenesis

我们建议购买一种独特的声学生物打印机CymatiXT（瑞士mimiX生物治疗公司，该技术的唯一供应商）。声诱导3D生物织物和形态发生是使用声波生成多细胞、空间协调的组织构建体的温和、快速且容易的方法。这种创新技术提供了克服传统方法的一些主要局限性的前景，以创建生理相关的体外模型和工程组织构建体。这项技术的独特性将推动3D生物织物在基础和应用研究方面的前沿。所要求的仪器将独特地支持和补充我们的创新研究计划的使命，以1）制定，表征和验证用于3D生物打印人类多能干细胞衍生组织的新型生物墨水; 2）使用尖端3D生物打印和人类多能干细胞技术开发组织工程模型; 3）通过提供能够忠实地再现体外人体生理学和疾病特征的组织工程3D模型来改变药物开发过程。它还支持我们计划的愿景，即生产和验证用于替代的工程组织和器官，并作为药物安全性和有效性测试的3D模型。该仪器将安装在Sainte Justine医院研究中心（CRCHUSJ）。这将使CRCHUSJ和蒙特利尔大学的所有感兴趣的用户都能够使用该仪器，这将产生重要的跨学科合作工作。申请人和用户的受训人员（HQP）将使用3D生物打印机获得实践培训，为他们提供其学科工作所需的市场技能。3D生物打印机和团队成员的实验室将成为吸引生物医学工程领域积极性高、合格的学生和研究人员的吸引点。通过收购这台3D生物打印机，我们的创新计划将彻底改变组织工程和器官生物打印，并具有在组织制造领域未来全球领导地位的巨大潜力。它还将为加拿大人带来社会、卫生和经济利益，包括加强对高素质人员的培训和提高他们的技能。该计划的好处将通过技术转让活动、知识产权许可、创建新的初创公司以及与我们的合作伙伴（包括加拿大国家研究理事会、蒙特利尔TransMedTech研究所、Axelys和MEDTEQ+等）的合作来实现。

We propose purchasing a unique acoustic bioprinter, CymatiXT (mimiX biotherapeutics, Switzerland, the only vendor of this technology). Sound-induced 3D biofabrication and morphogenesis is a gentle, fast, and easy method to generate multi-cellular, spatially orchestrated tissue constructs using sound waves. This innovative technology provides prospects to overcome some major limitations of conventional methods towards the creation of physiologically relevant in vitro models and engineered tissue constructs. The uniqueness of this technology will push the frontiers of 3D biofabrication both in fundamental and applied research. The requested instrument will uniquely support and complement the mission of our innovative research program to 1) formulate, characterize and validate novel bioinks for 3D bioprinting of human pluripotent stem cell-derived tissues; 2) develop tissue-engineered models using cutting-edge 3D bioprinting and human pluripotent stem cell technologies; 3) transform the drug development process by providing tissue-engineered 3D models capable of faithfully recapitulating the hallmarks of human physiology and disease in vitro. It also supports the vision of our program to produce and validate engineered tissues and organs for replacement and as 3D models for drug safety and efficacy testing. The instrument will be installed at the Research Center of the Sainte Justine Hospital (CRCHUSJ). This will enable all interested users in the CRCHUSJ and University of Montreal to have access to the instrument, which will generate significant interdisciplinary collaborative works. The 3D bioprinter will be used by trainees (HQP) of the applicants and users to acquire hands-on training that will provide them with the marketable skills required for jobs in their disciplines. The 3D bioprinter and team members' laboratories will become attraction poles for highly motivated and qualified students and researchers in biomedical engineering. By acquiring this 3D bioprinter, our innovative program will revolutionize tissue engineering and organ bioprinting and have the spectacular potential for future global leadership in tissue manufacturing. It will also generate social, health, and economic benefits for Canadians, including enhanced training and improved skills for highly qualified personnel. The benefits of this program will be realized by technology transfer activities, intellectual property licensing, creation of new start-up companies, and collaboration with our partners, including the National Research Council of Canada, Montreal TransMedTech Institute, Axelys, and MEDTEQ+, among others.