Vascular Optical Imaging

血管光学成像

• 批准号: CRC-2021-00091
• 负责人: Lesage, Frederic
• 金额: $ 14.57万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Canada Research Chairs
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=753977
• 关键词: Vascular; Optical; Imaging

The objective of the chair program is to pursue the development of innovative optical imaging technologies and state of the art simulations to investigate the causative role of vascular dysfunction in brain damage. Blood vessels form a plumbing network used to carry oxygen to tissue using red blood cells as a transporter. From a simple network in larger arterial segments to the complex topological structure of capillary beds, the vascular system plays a fundamental role in the delivery of oxygen molecules to cells. With age and disease, larger pipes in the system degrade both becoming more rigid and less responsive to hemodynamic stress while the smaller pipes (arterioles, capillaries) see connections and functional changes modifying their tissue footprint and their ability to deliver oxygen. First and foremost, impaired oxygen delivery affects the brain due to constant metabolic demand that needs to be met at every point in time to avoid damage. This chair program aims to develop novel technology to measure and accurately model microvascular function, oxygen delivery and potential associations with neural damage in complex and realistic environments. We will exploit combined 2/3-photon and Optical Coherence Tomography (OCT) microscopy imaging instruments to assess in vivo, how vascular properties modulate neural tissue oxygenation and neural death. With ground truth from in-vivo measures and ex-vivo structural information, we will then build and validate whole-brain first-principles vascular mechanistic models quantifying blood flow and oxygen delivery to tissue based on O2 advection/diffusion principles. These models will serve to simulate conditions associated with vascular dysfunction and allow modelling its impact on oxygen delivery and macroscopic signals measured with Magnetic Resonance Imaging (MRI). This will provide a unique tool to model the impact of microvascular dysfunction and to identify signatures of vascular diseases.With this research program we hope to develop technology that will help elucidate the role of vascular and micro-vascular network dysfunction in brain damage. With modelling developments, we aim to assess the manifestations/biomarker-signatures of these changes when measured with large clinical imaging instruments. Finally, new imaging technology developed to reach our goals is expected to provide instruments that can be disseminated and used beyond the investigations planned here.

主席项目的目标是追求创新光学成像技术的发展和最先进的模拟，以研究血管功能障碍在脑损伤中的致病作用。血管形成一个管道网络，利用红细胞作为转运体将氧气输送到组织中。从大动脉段的简单网络到毛细血管床的复杂拓扑结构，血管系统在将氧分子输送到细胞中起着重要作用。随着年龄和疾病的增长，系统中较大的管道会退化，变得更加坚硬，对血流动力学压力的反应也会减弱，而较小的管道（小动脉、毛细血管）的连接和功能变化会改变它们的组织足迹和输送氧气的能力。首先，氧气输送受损会影响大脑，因为在任何时候都需要满足持续的代谢需求，以避免损伤。该项目旨在开发新技术，以测量和准确模拟复杂和现实环境中的微血管功能、氧气输送以及与神经损伤的潜在关联。我们将利用联合2/3光子和光学相干断层扫描（OCT）显微镜成像仪器来评估体内血管特性如何调节神经组织氧合和神经死亡。根据体内测量和离体结构信息的基本事实，我们将建立并验证基于氧气平流/扩散原理的全脑第一性原理血管机制模型，量化血流和氧向组织的输送。这些模型将用于模拟与血管功能障碍相关的条件，并允许模拟其对氧输送和磁共振成像（MRI）测量的宏观信号的影响。这将提供一个独特的工具来模拟微血管功能障碍的影响，并确定血管疾病的特征。通过这个研究项目，我们希望开发出有助于阐明血管和微血管网络功能障碍在脑损伤中的作用的技术。随着模型的发展，我们的目标是在使用大型临床成像仪器测量时评估这些变化的表现/生物标志物特征。最后，为实现我们的目标而开发的新成像技术有望提供可以传播和使用的工具，超出这里计划的调查范围。