Scale Up and Optimization of the Fermentation Process for the Production of SARSCoV-2 RBD for Large Scale Serological Testing

用于大规模血清学检测的 SARSCoV-2 RBD 生产发酵工艺的扩大和优化

• 批准号: 567665-2021
• 负责人: Sheikhzadeh, MehdiM
• 金额: $ 5.46万
• 依托单位: Lambton College of Applied Arts and Technology
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761522
• 关键词: Scale; Up; Optimization; Fermentation; Process

The COVID-19 pandemic is unlike anything the world has seen in over a century, both in terms of global spread and economic impact. Caused by the infectious SARS-CoV-2 virus, the number of COVID-19 infections and deaths continue to rise. In particular, a major barrier to "flattening the curve" has been the availability of cheap, reliable and serologically-reactive sources of SARS-CoV-2 proteins for use in diagnostic testing. Suncor is Canada's premier integrated energy company, the fifth largest North American energy company and has a place on the global stage as one of the largest independent energy companies in the world. Suncor through this proposed project is continuing the work on a previous project funded by NSERC College and Community Social Innovation Fund that focused on facilitating a rapid test to identify people that have been exposed and become immune to the latest virus called SARS-CoV-2. The proposed project is a continuation of the work completed in the previous project and will focus on the optimization and scale up the fermentation process for the production of SARS-CoV-2 RBD for large scale serological testing and adapt the technology to a wider array of viruses. Successful completion of this project will allow up scale and optimization of the current process and prepare for commercialization. The success of this project will also help strengthen the Canadian bio-economy by developing a manufacturing process that can be implemented in Canada. An efficient and scaled-up approach to generate RBD protein for serological testing and other similar products is needed to drive new developments in viral testing.

COVID-19大流行无论在全球传播还是经济影响方面，都不同于世界上世纪以来的任何一次。由传染性SARS-CoV-2病毒引起的COVID-19感染和死亡人数持续上升。 特别是，“使曲线变平”的一个主要障碍是用于诊断测试的SARS-CoV-2蛋白质的廉价、可靠和血清学反应性来源的可用性。森科尔是加拿大首屈一指的综合能源公司，北美第五大能源公司，并在全球舞台上占有一席之地，是世界上最大的独立能源公司之一。 Suncor通过这个拟议的项目正在继续由NSERC学院和社区社会创新基金资助的先前项目的工作，该项目的重点是促进快速测试，以识别已暴露并对最新病毒SARS-CoV-2免疫的人。 拟议的项目是前一个项目中完成的工作的延续，将侧重于优化和扩大生产SARS-CoV-2 RBD的发酵过程，以进行大规模血清学测试，并使该技术适用于更广泛的病毒。该项目的成功完成将允许扩大和优化当前工艺，并为商业化做好准备。该项目的成功还将有助于通过开发可在加拿大实施的制造工艺来加强加拿大的生物经济。需要一种有效和规模化的方法来产生用于血清学检测和其他类似产品的RBD蛋白，以推动病毒检测的新发展。