Flow Cytometer for Advanced Cell marker Analysis

用于高级细胞标记物分析的流式细胞仪

• 批准号: RTI-2023-00223
• 负责人: Bendayan, Reina
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=762019
• 关键词: Flow; Cytometer; Advanced; Cell; marker

As cells develop and grow, they express different proteins that perform the functions required for life. For instance, cell development from one cell fate (such as a bone marrow stem cell) to another (such as an immunity-providing T cell) is concomitant with an increase in proteins known as cell markers of the new fate. Cell markers have been described for diverse cell fates, including those on specific organs (like liver or brain cells), diseases (such as cancer or arthritis), or stages of the cell cycle (actively replicating or dying). To perform analyses of cell marker expression, one requires the ability to separate a complex mixture of cells and probe each cell for the presence or absence of specific markers. This is best accomplished with the use of flow cytometry. Flow cytometry is an advanced technique used to detect and measure the presence of cell markers on individual cells, collecting information about the markers present on individual cells within populations of millions of cells. The observations captured by a flow cytometer give an accurate and detailed picture of the health, development, and functional states of complex mixtures of cells. At the Leslie Dan Faculty of Pharmacy at the University of Toronto, we use flow cytometry to help us characterize several cells, in particular, blood cells, liver cells, and brain cells, to better understand cell function that may translate to human health and disease. Currently, 10 different labs within our Faculty rely on a flow cytometer to advance their biology and biotechnology research. This research powers new insights into brain development, the control of drug and nutrient transporters, how diabetes impacts cell behaviours, and so much more. Flow cytometry is a powerful and unique technique considered the gold standard for cell marker analysis. As such, it plays a crucial role in our research and our highly qualified personnel training. Opportunities to train our students on a flow cytometer open our trainees to competitive future jobs such as scientist roles in biotech companies, product development and training specialists, researchers in academic and diagnostic facilities, and academic roles such as professors or lab course instructors. This training will enrich Canada's scientific talent and keep our students and industries globally competitive. Our existing flow cytometer has reached the end of its useful lifetime and must be replaced. Without a replacement purchased soon, our research will be sidelined since external, pay-for-use facilities are outside the scope of our research budgets. The purchase of a replacement flow cytometer will allow us to continue our important work and train the next generation of Canadian cell biologists.

随着细胞的发育和生长，它们表达不同的蛋白质，这些蛋白质执行生命所需的功能。例如，从一种细胞命运（如骨髓干细胞）到另一种细胞命运（如提供免疫力的T细胞）的细胞发育伴随着称为新命运细胞标志物的蛋白质的增加。细胞标记物已被描述为不同的细胞命运，包括特定器官（如肝或脑细胞），疾病（如癌症或关节炎）或细胞周期阶段（活跃复制或死亡）。为了进行细胞标志物表达的分析，需要分离细胞的复杂混合物并探测每个细胞是否存在特定标志物的能力。这最好通过使用流式细胞术来完成。流式细胞术是一种先进的技术，用于检测和测量单个细胞上细胞标志物的存在，收集关于数百万细胞群体中单个细胞上存在的标志物的信息。通过流式细胞仪捕获的观察结果可以准确而详细地了解复杂细胞混合物的健康、发育和功能状态。在多伦多大学的Leslie Dan药学院，我们使用流式细胞术来帮助我们表征几种细胞，特别是血细胞，肝细胞和脑细胞，以更好地了解可能转化为人类健康和疾病的细胞功能。目前，我们学院内的10个不同的实验室依靠流式细胞仪来推进他们的生物学和生物技术研究。这项研究为大脑发育，药物和营养转运蛋白的控制，糖尿病如何影响细胞行为等提供了新的见解。流式细胞术是一种强大而独特的技术，被认为是细胞标志物分析的金标准。因此，它在我们的研究和高素质人才培养中发挥着至关重要的作用。培训我们的学生在流式细胞仪的机会打开我们的学员竞争未来的工作，如在生物技术公司，产品开发和培训专家，在学术和诊断设施的研究人员，科学家的角色，如教授或实验室课程讲师学术角色。这项培训将丰富加拿大的科学人才，并保持我们的学生和行业的全球竞争力。我们现有的流式细胞仪已达到其使用寿命，必须更换。如果不尽快购买替代品，我们的研究将被搁置，因为外部付费使用设施不在我们的研究预算范围内。购买替换流式细胞仪将使我们能够继续我们的重要工作，并培养下一代加拿大细胞生物学家。