Use of multi-omic approaches to enable non-lethal biological effects monitoring of wild fish

我的研究计划的长期愿景是促进对淡水鱼和其他脊椎动物血浆功能的理解，并建立血浆在环境影响监测计划中用作非致命诊断工具的潜力。该研究计划将开发和演示如何将高通量分子方法和生物信息学软件工具的使用纳入环境监测和评估计划的政策和程序。这些方法将能够确定暴露于改变脂质代谢的水生污染物，并能够预测淡水鱼的长期健康结果。我的研究计划将解决三个相互关联的目标：1。开发新的蛋白质组学生物信息学工具，利用蛋白质组学方法对目前未测序的野生鱼类物种进行有效的非致死性监测2.了解胁迫下鱼类血浆中分子表达的生理原因，以及与代谢和生物能量功能特别相关的分子反应如何受到其自然环境中挑战的影响-其目的是建立“正常”3.通过检测化学物质如调脂药物和全氟化学物质的分子效应，然后确定污染物暴露所特有的蛋白质和代谢物生物标志物的独特集合，来机械地表征污染物如何影响代谢和生物能量学。这项研究还将有助于了解全氟污染物的影响，这些污染物存在于北美饮用水中，并已知会在人类和野生动物体内积累。此外，这些信息将有助于减少在研究中使用动物，并将特别有助于保护加拿大的濒危和受威胁物种。这项研究还将有助于培养11名环境科学、分子生物学和仪器分析方面的高素质人才。这项工作将使加拿大人在经济上受益，增加社会与自然和野生动物互动的能力，同时享受运动钓鱼、露营和在湖泊和河流中游泳等活动。

The long-term vision for my research program is to advance understanding of blood plasma function in freshwater fish and other vertebrate animals and to establish the potential for plasma to be used as a non-lethal diagnostic tool in environmental effects monitoring programs. This research program will develop and demonstrate how to incorporate the use of high throughput molecular methods and bioinformatic software tools into environmental monitoring and assessment program policies and procedures. These methods will be able to identify exposure to aquatic contaminants that alter lipid metabolism and will be capable of predicting long-term health outcomes in freshwater fish. My research program will address three inter-related objectives:  1. To develop novel proteogenomic bioinformatics tools that enable the efficient non-lethal monitoring of currently un-sequenced wild fish species using proteomic approaches 2.Understand the physiological causes of molecular expression in fish blood plasma under stress, and how molecular responses that are specifically related to metabolic and bioenergetic functions are affected by challenges in their natural environment - the purpose of this is to establish "normal" ranges of molecules under realistic stressful scenarios 3.Mechanistically characterize how contaminants affect metabolism and bioenergetics by examining the molecular effects of chemicals such as lipid regulating drugs and perfluorinated chemicals and then identifying unique sets of protein and metabolite biomarkers that are specific to contaminant exposure This research program will increase our understanding of the role of blood plasma in vertebrate physiology and function. This research will also contribute to the body of knowledge about the effects of perfluorinated contaminants that are present in North American drinking water and known to accumulate in humans and wildlife. Additionally, the information will help to reduce the use of animals in research and will be especially useful in protecting endangered and threatened species in Canada. This research will also help to train 11 highly qualified personnel in environmental science, molecular biology, and instrumental analysis. This body of work will benefit Canadian economically by increasing society's ability to interact with nature and wildlife while they enjoy activities such as sport-fishing, camping, and swimming in lakes and rivers.