大体积工程化脂肪瓣的体内诱导及调控机制

以乳房再造为代表的大体积软组织缺损修复是整形外科面临的最大挑战，经典的手术治疗难以摆脱“拆东墙补西墙”的尴尬。组织再生诱导技术为软组织缺损后再生提供了可能。本课题组改进了经典的组织工程室技术，通过先期启动体内创伤/炎症因素，辅以外悬吊力学环境诱导，成功地实现了大体积脂肪再生的诱导，并初步揭示了脂肪组织内“力学-化学”信号的转化模式及其调控机制。据此我们推测：虽然先期的创伤/炎症是再生的启动因素，但持续性的力学刺激是维持工程室内脂肪再生诱导的核心。本研究拟采用可控力学细胞培养、基因芯片、骨髓移植等方法，重点研究生物力学在脂肪再生诱导中的具体靶细胞以及信号转导模式，深入探讨 “力学-化学”信号对脂肪再生的诱导及调控机制，为大体积软组织缺损再生的诱导提供理论依据。

Repair of large volume soft tissue defect, especially breast reconstruction is the biggest challenge for plastic surgeons, for donor site morbidity is inevitable with surgery. Tissue regeneration offers a new solution to the reconstructive surgery. Based on the studies of traditional tissue engineering chamber technique, our research group first developed this novel adipose regeneration procedure to generate large-volume adipose flap. This process was initated by trauma and inflammation created by surgery and then maintained by in vitro mechanical force. Results also suggested that though trauma/inflammation environment was important at the beginning, the biochemical signal transferred from mechanical force played a key role during this process. However, the mechanism is not clear. The following researches will focus on the transformation of the mechanical force to biochemical signal using cytobiology, microarray analysis, bone marrow transplantation and histomorphology methods, clarifying the role that mechanical-biochemical signal plays in the adipose regeneration process. This research is believed to be helpful for better understanding the regeneration process of adipose tissue.