选择适宜的有机化合物作稳定剂，采用化学物理方法制备稳定的不同形貌和尺寸的银/氧化石墨烯等纳米酶溶胶；采用光谱，电镜，能谱等技术对纳米酶溶胶进行表征。筛选高选择性高灵敏的核酸适配体反应和灵敏的纳米酶催化微粒反应；研究纳米酶催化微粒反应的共振瑞利散射（RRS）光谱特性及核酸适配体对纳米酶催化活性的影响，探索靶分子-核酸适配体反应对纳米酶活性的调控，构建适配体调控纳米酶催化RRS探针分析新体系。优化分析条件，发展简便快速灵敏测定重金属离子靶分子的适配体调控纳米酶催化-RRS光谱分析新方法，并用于环境样品中重金属污染等的分析；研究液相纳米酶表面催化微粒反应机理。

Stable silver/oxide graphene(GO) nanoparticle and its nanoalloy nanoenzyme sols in differen size and morphology were prepared by chemical procedure using suitable orgnic compound as stabile reagent. The nanosilver/GO and its nanoalloy nanoenzyme colloides were characterized by spectral, electron microscopy and energy spectrum and so on. Highly sensitive and selective aptamer reaction and sensitve nanoenzyme catalytic particle analytical reaction were screened to fabricate analytical system of aptamer-controlled nanoenzyme particle reaction, and the regulation of nanoenzyme activity was studied by means of the target molecule of analyte and aptamer reaction. The influence of various factors on the aptamer reaction and the nanoenzyme were considered, and aptamer-controlled nanoenzyme catalytic RRS method was developed for the determination of trace metal ions in water and foods samples of Guangxi province.The mechanism of liquid nanoenzyme surface catalytic particle reaction was studied.