顺铂虽广泛用于实体瘤的治疗，但毒副作用和耐药性严重影响其疗效。本研究拟用食品成分白杨素联合顺铂，在动物和体外细胞试验基础上，从整体、细胞、分子水平入手，观察白杨素能否促进顺铂介导的肿瘤细胞凋亡、提高顺铂的抗肿瘤活性；并在已有研究基础上以转录因子P53为切入点，在mRNA、蛋白质水平观察其表达水平和稳定性的变化趋势，沿p53信号通路观察其上游NF-κB、JNKs等调控蛋白、下游凋亡相关蛋白和MDM2等负调控因子的变化规律，进而阐述p53在白杨素提高顺铂对肿瘤细胞毒性、增强抗肿瘤活性中的作用及白杨素在p53从活化→入核→DNA结合→翻译→表达的整个转录调控过程中的可能的作用位点，为克服顺铂在肿瘤治疗中的耐药性和低敏感性问题提供新的思路，为白杨素在肿瘤防治中的应用提供更广泛的科学依据

Cisplatin is widely used in the treatment of solid tumor, .while the major barriers limiting its applicaion and efficacy are toxicity and resistance.In this project, the combinative effects of chrysin and cisplatin are studied to find out whether cisplatin-induced anticancer activity can be potentiated by chrysin using methods in vivo and in vitro. We have determined that chrysin could sensitize the cell death-induced by cisplatin in cancer cells(Hep G2 and BGC-823). On the basis of present researches, we are going to focus on transcription factor p53 to observe the trendency of its expression and stability, and to anlyse the regulating rules of the up- and downstream proteins along p53 singnaling pathway,such as NF-κB，JNK ,apoptotic protein and MDM2, trying to explore the role of p53 in the cotreatment and the molecular point which chrysin acts on along the line of activation→translocation to nuclear→DNA binding→translation→expression. In this research, we endeavor to find out a approch to overcome the drug-resistance and low-sensitivation of cisplatin from a new perspective and pave the way for chrysin to be applied in the anticancer treatment.