前期发现 EBV-miR-BART7可能调控宿主基因PTEN和AnxA1表达，与鼻咽癌EMT和侵袭转移有关。为验证假说，本项目首先探讨EBV-miR-BART7在临床鼻咽癌侵袭转移中作用，同时过表达或沉默EBV-miR-BART7，从体内外实验证实其在鼻咽癌中生物学功能（EMT、侵袭转移）。然后，通过鼻咽癌细胞中Luciferase报告系统、EBV-miR-BART7表达或沉默后靶基因表达分析，结合临床标本验证，明确EBV-miR-BART7在鼻咽癌中确实靶向PTEN和AnxA1。最后，探讨EBV-miR-BART7通过PTEN、AnxA1影响下游信号诱导Actin重排和EMT并促进鼻咽癌侵袭转移的分子机制以及该机制在EBV（+）鼻咽癌细胞中所能发挥的作用，并阐明EBV-miR-BART7与PTEN/Akt间可能存在的反馈机制。据此提出EBV miRNA调控宿主基因促鼻咽癌侵袭转移新机制。

Our previous study initially found that EBV-miR-BART7 may be involved in EMT，invasion and metastasis of nasopharyngeal carcinoma by directly regulating human host`s PTEN and AnxA1 expression.To illustrate our findings, this project intends to firstly carry out the investigation of clinical samples by in situ hybridization, combined with clinical and pathological data, to explore the correlation between EBV-miR-BART7 and the invasion and metastasis of nasopharyngeal carcinoma. Moreover, in vitro and in vivo experiments will be used to validate the biological functions of EBV-miR-BART7 in nasopharyngeal carcinoma ( including invasion\metastasis, F-actin rearrangement and EMT).Then, Luciferase reporter analyses on nasopharyngeal carcinoma cell line, EBV-miR-BART7 overexpression and silencing experiments and correlation analysis in clinical samples will be appled to define that EBV-miR-BART7 can directly regulate PTEN and AnxA1 in nasopharyngeal carcinoma. Furthermore, this project continues to explore how EBV-miR-BART7 regulates PTEN, AnxA1`s downstream signals and thereby induces Actin rearrangement and EMT promoting the invasion and metastasis of nasopharyngeal carcinoma. Finally, the possible existence ot positive feedback regulation between EBV-miR-BART7 and PTEN/Akt is going to be explored. The project is expected to present a viral miRNA -mediated regulation mechanism of NPC invasion and metastasis.