基于多位点免疫脂质磁球技术的肿瘤单细胞分选鉴定系统的构建及在儿童脑胶质瘤诊断方面的应用

本项目将基于已有的多功能免疫脂质磁球（EpCAM、GFAP、SYN、VIM等大分子免疫磁球，具有类细胞双层膜结构的高细胞亲和分选能力）及免疫荧光技术，对不同的儿童脑瘤类型（髓母神经瘤、室管膜肿瘤、PNET等）的患者脑脊液及血液中的肿瘤细胞进行分离鉴定；在对获得的肿瘤单细胞进行计数的基础上，结合肿瘤单细胞的免疫组化/Fish信息，及肿瘤单细胞的基因突变和全基因组测试数据，结合临床特征（病理、肿瘤分级分期、化疗敏感性、耐药性、放疗敏感性、预后预测等），探寻肿瘤细胞的异质性特征，并最终对患者进行个体化的诊断评估分析，指导临床治疗。项目的进行将开拓一种全新的适合不同肿瘤的液体活检单细胞分析技术，具有重大的临床实用价值。

The lipid immune magnetic ball was adopted in this project for immunofluorescence glioma circulating tumor cell separation identification system, and were used for single-cell sequencing in patients with brain glioma in children in real-time monitoring of tumor cells through the change of the number of tumor cells in blood and cerebrospinal fluid. Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. We also want to analysis an integrated process to isolate, qualify and sequence whole exomes of CTC with high fidelity, using a census-based sequencing strategy. Combined with the clinical data of patients, pathology and imaging data, children's blood and cerebrospinal fluid in patients with cerebral glioma tumor cell number, the sequencing results and clinical information (tumor classification stage, chemotherapy sensitivity, resistance, radiation sensitivity, prognosis prediction) will be explored. The relationship between CTC data and clinical feature will be used to guide the clinical doctor's judgment and individualized treatment.