菌核病是由核盘菌侵染引起的一种重要植物病害，它严重危害了油菜、甘蓝等十字花科农作物的生产。项目组前期通过对甘蓝接种前后的茎杆转录组测序分析，发现铜离子代谢相关基因和代谢途径参与了寄主和核盘菌互作。本项目拟调查油菜铜离子分子伴侣基因BnCCH的DNA结构变异及其对菌核病抗性影响，明确BnCCH 基因响应核盘菌侵染的顺式元件和相应的反式因子，解析油菜BnCCH 基因参与的菌核病抗病相关网络，开发与菌核病抗性相关的功能标记。该项目的开展对于揭示寄主和核盘菌互作机制，和油菜的抗病育种具有理论和实践意义。

Sclerotinia stem rot is one of important diseases, which causes serious loss of production in Brassica crops, such as B. napus and B. oleracea. By transcriptome sequencing of stem after and before inoculation of sclerotinia in B. oleracea, we found the genes and pathways related with cooper iron were involved with the interaction between host and sclerotinia in previous research. In this project we will investigate structural and functional variance of BnCCH in natural population of B. naps, trans- and cis- interaction of BnCCH and resistance nets associated with BnCCH. It will be helpful for understanding the interaction mechanism between host and sclerotinia, and promoting resistance breeding by development of functional markers in B. naps.