可视化快速检测技术的研究在食品安全、环境监测以及临床诊断等领域得到高度关注，本项目拟开展光致变色功能化贵金属纳米探针增强可视化快速检测方法研究。分别以二芳基乙烯和螺吡喃为母体，根据待测物性质拟基于多重氢键、螯合作用、衍生化等识别机制，设计合成具有比色荧光双响应及高特异性的光致变色支链，采用“一锅法”构建光致变色功能化贵金属纳米探针；研究探针对目标物的识别机理，探索内核贵金属纳米粒子作用外壳有机分子所产生表面增强荧光及拉曼效应的规律，通过调控连接支链长度及贵金属纳米粒子的粒径优化探针性能；发展小分子化合物及离子的快速灵敏、准确可靠的可视化检测方法；实现食品、环境及生物等复杂样品中痕量组分的可视化快速检测。研究成果将丰富和拓展可视化快速检测技术的应用范围，为复杂样品中痕量有毒有害物质的快速筛查、现场检测提供技术支撑。

The research of rapid visual detection (RVD) technique have been highly concerned in the field of food safety, environmental mornitoring and clinical diagnosis. In this proposal, studies concerning RVD technique are scheduled, which are based on noble metal nano-probe covered with photochromic monomer/polymer. This program aims to develop a multi-response, sensitive and selective core-shell nano-probe system, and includes specific content as follow: (1) to design and synthesize probes with a functionalized photochromic shell featured dual-response and selectivity, and a nano-core highlighted surface enhancement effect. Specifically, according on individual recognition mechanism like mulriple hydrogen bond, chelation, derivation etc., the shell would be modified and functionalized from diarylethene-based or spiropyran-based template and further covered on the nano-core by using ‘one-pot’ reaction. (2) to study response and surface enhancement mechanism, and to optimize recognizing performance by varying chain-length of monomer/polymer and nano-particle diameter. (3) to establish RVD methods towards trace small molecular compounds and ions in complex systems. Anticipatory accomplishments would greatly enrich and expand RVD technique, providing theoretical and technical support for rapid field detection, and trace detection of toxic and harmful substances in complex samples.