抗精噁唑禾草灵的多花黑麦草是严重威胁我国小麦安全生产的重要恶性杂草之一。代谢抗性作为最重要、最常见的非靶标抗性机理，其威胁相比靶标抗性更大但研究进展缓慢。本项目申请者前期研究发现：细胞色素P450基因CYP81A1参与了多花黑麦草对精噁唑禾草灵的代谢解毒，该基因过量表达后可导致水稻愈伤组织对精噁唑禾草灵产生抗性，但是其精准的机理尚不清楚。申请者拟继续以多花黑麦草的抗性生物型与对应的敏感型为研究材料，通过生物信息学、分子生物学与代谢组学等多学科的交叉研究方法，首先以分子模拟、异源表达明确多花黑麦草CYP81A1基因与精噁唑禾草灵抗性的关系，其次通过转基因水稻佐证多花黑麦草来源CYP81A1基因的功能，最后探明多花黑麦草CYP81A1基因参与解毒精噁唑禾草灵的代谢通路。预期研究结果可揭示CYP81A1过表达介导的多花黑麦草代谢抗性的机理，发现杂草代谢抗性的新机理，应用前景广阔。

Wheat is an important crop in China. Lolium multiflorum is a serious gramineous weed in wheat fields in China. Fenoxaprop is one of the most widely used aryloxyphenoxypropionate herbicides (AOPPs) in China to control annual gramineous weeds in wheat fields. However, its controlling efficiency has been decreasing with the contiuous application in fields. Currently, target-site resistance and metabolic resistance are recognized as the main mechanism of resistance. In our previous research, target-site resistance were excluded from one fenoxaprop-resistant L. multiflorum. RNA-seq analysis and a series of phenotype/genotype validation experiments reveal one cytochrome P450 contig CYP81A1 higher expressed in the R than S L. multiflorum plants. Rice calli overexpressing the CYP81A1 gene is resistant to fenoxaprop. In the present study, we aim to study the bioinformatics molecular modeling of CYP81A1 gene, express the CYP81A1 protein in E.coli, and overexpress CYP81A1 gene in rice seedlings, performe metabolomics analysis of CYP81A1 transgenic rice seedlings. The results of this study can reveal a novel metabolic gene that confers resistance to fenoxaprop, and provide the key information to the management of fenoxaprop-resistant L. multiflorum.