创面感染一直是骨科领域长期致力解决的难题。前期研究认为：感染是细菌与宿主体内环境相互作用的复杂过程，宿主体内渗透压、pH、温度等环境信号能诱导细菌致病基因表达，对细菌致病过程起重要作用。我们前期研究也发现：负压封闭环境可引起金葡菌致病基因及毒力因子表达变化，但机制尚不明确。附属基因调节子(agr)系统是一种重要的感应信号和毒力因子调节系统，研究表明几乎所有金葡菌毒力因子均受到agr调节。我们前期研究发现：负压封闭环境能抑制金葡菌agr系统表达。提示：负压封闭环境可能通过调控agr系统影响金葡菌毒力因子表达，进而起到控制感染作用。据此提出本课题设计：①研究不同负压模式下细菌毒力因子表达与变化趋势；②负压环境影响金葡菌致病基因及上游调控系统关键位点筛选；③通过基因敲除技术验证上游关键调控基因对细菌毒力与细菌侵袭行为影响。以期深刻揭示负压环境调控金葡菌毒力因子表达机制，为控制细菌感染奠定基础。

Wound infection is a long challenged problem in orthopedics area.Previous study indicated that infection is a complicated procedure of the interaction between bacteria and host internal environment.Host osmotic pressure、PH and temperature inducing the express of bacteria virulence gene made a contibution to infection procedure.Our previous study discovered that negative pressure environment could cause a change of bacteria virulence gene and factors,however the mechanism is unclear up to now.Accessory gene regulator is an important sensing signal and virulence factors regulating system.Studies indicated that nearly all Staphylococcus aureus virulence factors were regulated by agr system.Our previous study found that negative pressure environment could inhibit express of Staphylococcus aureus agr system.This meant that negative pressure environment may change Staphylococcus aureus virulence factor expression by regulating the agr system,then promote a control of infection.In view of the above,we propose this research:①The trend of the bacteria virulence factor expression changing under different negative pressure mode.②Negative pressure's impact on the Staphylococcus aureus virulence gene and the genetic screening from the regulating system③Testing the the key gene's impact on bacteria virulence and invasion through gene knock-out technique.Revealing the mechanism of expession of Staphylococcus aureus virulence factors made a foundation of infection control.