乳汁管是植物体内的一种分泌结构，其乳汁中含有许多特有的代谢产物，在工业和医药上有广阔的应用前景，因此研究乳汁管的发育调控具有重要的理论实践意义。甘遂为我国特有植物，传统中药，全株具有无节分枝乳汁管—一个单细胞体系。我们检测了细胞自噬在甘遂乳汁管发育过程中的发生，发现乳汁管可通过形成自噬体，并与类溶酶体小泡融合进行原生质的降解。但是，目前关于乳汁管发育过程中自噬的发生模式，乳汁管细胞自噬发生机理还不清楚。故本项目拟以甘遂为材料，在茉莉酸甲酯不同时间诱导的甘遂比较转录组和乳汁的蛋白质组学基础上，利用实时荧光PCR、激光共聚焦显微镜以及透射电子显微镜技术，通过对茉莉酸甲酯、细胞自噬抑制剂分别处理后，乳汁管发育过程中自噬相关基因的表达分析及自噬发生过程的观察，阐明乳汁管发育过程中细胞自噬的发生模式，再利用酵母双杂交技术，获得目标自噬相关蛋白的互作网络，从而为乳汁管发生发育的调控研究提供理论基础。

The laticifer is a specialized secretory structure in plants, and its milk contains many unique metabolites, such as natural rubber, cardenolides, alkaloids and terpenes, which have wide application in industry and medicine. Euphorbia kansui Liou is a unique species to China, a traditional Chinese Medicine, and has non-articulated branched laticifers in whole plant. We detected that autophagy involved in cytoplasmic degradation through directly engulfing cytocol and organelles by autophagosomes and then fusing with lysosome-like vesicles during the development of laticifers in Euphorbia kansui. However, at present, whether the central vacuole is involved in autophagy during the development of laticifers, the mechanism of autophagy in laticifers is not clear. Therefore, this project intends to, based on the transcriptome and latex proteome of Euphorbia kansui response to methyl jasmonate (MeJA), through the differential expression analysis of autophagy related genes, autophagy process observation during the laticifer development after treatment of MeJA and autophagy inhibitors using qRT-PCR, laser confocal microscopy and transmission electron microscopy, reveal the occurrence pattern and molecular mechanism of autophagy in the process of laticifer development. Finally, using yeast two-hybrid technique to search the interaction network of the target autophagy related protein, thus providing a theoretical basis for the regulation of laticifer development.