番茄果实成熟是涉及表观形态、生理和生化变化的复杂遗传性状，决定着果实采后的品质及贮藏特性，其中调控番茄果实成熟的重要转录因子LeSPL-CNR在该过程中起着关键调控作用，但其具体的调控机制尚不明确。因此，本研究将分别利用酵母单杂交、酵母双杂交及染色质免疫共沉淀结合深度测序（ChIP-seq）技术筛选LeSPL-CNR基因的上游调控因子、LeSPL-CNR蛋白的互作因子以及下游调控靶标，并利用病毒诱导的基因沉默（VIGS）及病毒诱导的基因互补（VIGC）技术对部分关键靶标基因的生物学功能进行验证，进而构建出番茄果实成熟过程中以LeSPL-CNR为中心的局部转录调控网络。研究成果将揭示LeSPL-CNR蛋白对番茄成熟和果实品质形成的生物学意义，促进番茄种质资源的鉴定及深度开发，为通过分子遗传育种途径培育优质、耐贮或专用番茄品种提供理论基础。

Tomato fruit ripening is a complex genetic trait correlating with notable fruit phenotypic, physiological and biochemical changes. It decides the postharvest qualities and storage characteristics of fruit. During ripening process, transcription factor LeSPL-CNR plays an important role; however, the exactly regulatory mechanism is not clear. Therefore, in this project, we propose to find out some important upstream regulatory factors of LeSPL-CNR gene, interaction factors and targeted genes of LeSPL-CNR protein via yeast one hybrid system, yeast two hybrid system, chromatin immunoprecipitation and massively parallel sequencing (ChIP-seq). Furthermore, the biological functions of acquired targeted genes are identified by virus-induced gene silence (VIGS) and virus-induced gene complementation (VIGC). Finally, a local transcription regulatory network around LeSPL-CNR for tomato ripening will be made. These results will illustrate the biological significance of LeSPL-CNR protein for tomato ripening and fruit quality forming, promote the identification and deeply development of the tomato germplasm resources, and provide theoretical foundation for getting better quality, storable or specialized tomato cultivars through molecular breeding.