典型甘油醚类化合物及其衍生物的平面波导荧光免疫分析方法构建与环境行为研究

双酚A二缩水甘油醚(BADGE)、酚醛清漆甘油醚(NOGE)及其衍生物是一类在生活与工业生产中广泛使用的典型甘油醚类化合物，具有内分泌干扰效应、细胞与基因毒性。这类物质被排放到环境中，将导致严重的环境污染问题。然而，目前其污染特征与环境风险仍不明确，方法学是制约这一问题的关键因素。现有的仪器分析法（SPE-LC/MS/MS）因前处理繁琐、分析时间长等不足，无法在较短时间内通过大量样本的检测得出规律性的结论。因此，发展简单快速的免疫分析方法极其必要。本项目拟分别制备可识别BADGE、NOGE及其衍生物的单克隆抗体，再以荧光试剂标记，构建前处理简单、灵敏度高、稳定性好的平面波导荧光免疫传感器，对污染物进行快速分析；通过高频次、多样品、长期的区域采样与分析，系统研究这些化合物在典型区域环境中的分布、迁移及生物富集过程，为进一步深入了解此类污染物的环境暴露风险并制定有关防控对策提供科学依据。

Bisphenol A diglycidyl ether (BADGE), novolac glycidyl ether (NOGE) and their derivatives are typical glyceryl ethers that are widely used in life and industrial productions. Studies reported endocrine-disrupting, genotoxic and cytotoxic potentials of the containments, and the chemicals could be discharged to environments and result in serious environmental problems. However, up to date, the environmental distribution and ecological risk of the pollutants are not clear, which is mainly due to lack of the suitable analytical methods. Currently the traditional instrument approaches suffer from complicated procedures in sample pretreatment, and time consuming to pre-concentrate and separate targets from matrices, as a consequence, the systematic results can not be obtained after measurement of massive environmental samples within a short time. Therefore, it is necessary to develop a simple and rapid immunoassay to monitor the organic containments. In the project we will design and synthetize a series of haptens, based on this, monoclonal antibodies against these compounds can be produced, after labelled by fluorescent reagent, a quick, simple, sensitive planar waveguide fluorescence biosensing system with good stability and high tolerance to numerous matrix interferents could be established for analysis of these chemicals from a large number of different environmental, biological and human samples in some typical regions. The distribution, transportation and bioaccumulation of these chemicals in contaminated regions will be studied in detail, our results will provide a scientific basis for further in-depth understanding of the environmental and health risks of these pollutants.