环境中四溴双酚A及其典型衍生物/副产物 的生物分析方法、污染特征与环境行为研究

四溴双酚A(TBBPA)是一种具有生殖毒性的内分泌干扰物，其衍生物和生产过程中产生的副产物可能具有更强毒性，是潜在的持久性污染物，可能会带来严重的健康危害和环境污染问题。然而，目前仅有极少的针对个别衍生物/副产物的仪器分析方法，而且存在仪器昂贵、测试成本高、分析时间长、前处理复杂等不足，限制了对此类污染物的系统研究。本项目拟针对TBBPA及其3种典型衍生物和对应的4种副产物，分别制备4种可识别TBBPA及其衍生物的单克隆抗体、4种可识别TBBPA副产物的抗体。在此基础上，建立多标记、高通量、高灵敏、快速的时间分辨荧光免疫分析方法，并对典型区域的环境样品、生物样品、人体样本进行分析，系统研究这类化合物的污染水平及特征，揭示其在不同植物和动物体内的累积差异，明确其来源、传输及生物富集规律，了解其在污染区域人群中的暴露途径与浓度水平，为进一步评估这些化合物对普通人群的环境暴露风险提供科学依据。

Tetrabromobisphenol A (TBBPA) is one of endocrinedisruptors with reproductive toxicity, some TBBPA derivatives and their byproducts may have more toxic effects, which was considered as potentially persistent organic pollutants, and the pollution and risks caused by these containments gradually become serious environmental problems and health hazards. However, currently only a few instrumental analytical methods were established for determination of TBBPA, and several derivatives or byproducts, and they suffered from expensive instruments, great costs for samples analysis, time consuming to pre-concentrate and separate targets from matrices, complicated procedures in sample pretreatment, all of which caused the lack of systematic research for these organic pollutants. Therefore, this project will be focused on TBBPA, three typical derivatives of TBBPA and four corresponding byproducts. Four monoclonal antibodies against TBBPA and three typical derivatives of TBBPA will be produced, and four specific antibodies recognizing corresponding byproducts also be generated, based on which, multiply targeted, high-throughput, quick, sensitive time-resolved fluoroimmunoassay could be developed for analysis of these chemicals from a large number of different environmental, biological and human samples, as a consequence of this, the distribution, pattern, transformation and bioaccumulation of these chemicals in contaminated regions will be studied in detail, and their source, transmission and exposure pathways will also be discussed. The results of this project will provide a scientific basis for further in-depth understanding of the environmental and health risks of these containments.