具有生物正交性的新化学报告基团的发展对于糖链的标记具有重要的意义。本项目拟将无取代的环丙烯基团引入糖链并进行代谢标记，考察该报告基团的稳定性、与四嗪类化合物探针进行反电性Diels-Alder反应的速率常数、代谢效率等，以期发现适合于糖链标记、性能更好的化学报告基团。

The development of new chemical reporters with bioorthogonal reactions is very important to glycan labelling. In this project, we will introduce the unsubstituted cyclopropenyl group as a chemical reporter to monosaccharide moieties and perform the Metabolic Oligosaccharide Engineering. We will investigate the stability of this chemical reporter, the rate constant of its inverse electron-demand Diels–Alder reaction with tetrazine probes, and its efficiency of metabolism. We hope to find better chemical reporter for glycan labelling.