兽药是重要的危害食品安全的化学污染物，动物性食品中的兽药多残留检测一直是食品安全检测领域的热点和难点。针对传统免疫分析方法难以同步定量检测多种不同种类兽药残留的技术瓶颈，本项目拟通过流式荧光免疫分析技术作为突破口，解决同时检测四种不同种类兽药多残留检测难题。本研究拟以荧光编码微球做免疫分析载体，利用不同种类荧光材料标记兽药抗原、抗体作为荧光检测探针，优化配对荧光编码微球与荧光标记物组合，在液相流体反应环境中进行多种兽药小分子抗原、抗体的竞争性免疫反应，建立多色流式荧光免疫分析方法，通过流式细胞仪同步定量分析特定荧光编码微球载体上荧光探针的荧光强度，同步定量检测动物性食品中磺胺类、喹诺酮类、大环内酯类和林可酰胺类等四类兽药残留。本研究可丰富现有的化学污染物多残留免疫检测技术，为动物性食品中兽药多残留免疫分析方法的设计、改进和发展提供必要的指导信息和新思路，具有重要理论意义和应用价值。

Food contamination caused by veterinary drugs is a world-wide public health concern. Multi-analyte immunoassay for simultaneously detecting multiple veterinary drugs in foods is in urgent need to solve the food safety issues in China. Traditional immunoassays usually focus on detecting single analyte or multiple analytes with similar chemical structures. Compared with parallel single-analyte immunoassay methods, the multi-analyte immunoassay offers high sample throughput, and could improve assay efficiency. The flow cytometry based multiplexed assay is an inherently and potentially promising analytical method to solve the bottleneck of multiple veterinary drug residue analysis. In the project we proposed, the multi-analyte profiling technology based multiple color fluorescence-encoded microsphere suspension array could be developed for simultaneously detecting multiple veterinary drug residues in food of animal origin. On the base of fluorescence-encoded microspheres, a new flow cytometry based multiplexed assay with traditional fluorescent dyes and novel fluorescent nanoparticles will also be developed for quantitatively determination of sulfonamides, quinolones, macrolides and lincosamides residues in foods of animal origin. After combining flow cytometry technology and fluorescent immunoassay, the flow cytometry based multiplexed assay could enrich the existing immunological analytical methods for chemical contaminants residues in foods of animal origin. The implement of the project would provide useful information for designing and improving new immunoassays for multiple chemical contaminates residue analysis.