花生过敏病情凶险，甚至危及生命，其原因尚未有明确报道。花生中主要过敏原结构复杂多样，其线性表位的研究已经比较清楚，但并不是其严重致敏性的主要原因。构象性表位是过敏原的重要组成，我们推测构象表位在花生的致敏过程中发挥重要作用，可能是花生过敏原高致敏性的主要原因。截止目前花生中主要过敏原构象表位的研究报道缺乏，构象表位表征困难，阻碍了对花生过敏原构象表位和致敏性的深入研究。花生中的主要过敏原分属于六个蛋白质家族中，其结构和功能差别较大。本项目拟通过突变、复原突变和原核表达的方法，构建花生中不同过敏原家族代表性过敏原的IgE构象表位的逆转突变体，通过人体血清学试验验证并鉴定出过敏原的构象表位。最后利用动物模型对所鉴定的构象表位进行验证，并解析花生中不同蛋白家族过敏原构象表位的特异性，阐明花生中不同过敏原家族过敏原构象表位在花生高致敏反应中的作用机制。

Peanut allergy is life-threatening, but the reason of high allergenicity of peanut allergens is not clear yet. Conformational epitopes are important component of the allergen epitope, and the conformational epitope may play an important role in peanut allergy. Peanut allergens are complex and diverse in structure, and their conformational epitopes may be family-specific which may indispensable for the high sensitization of peanut allergens. However, the lack of information on conformational epitopes and the difficulty in characterizing conformational epitopes have hindered the in-depth study of the allergenicity of peanut allergens.This project intends to construct reversal mutants of IgE conformation epitopes of six representative allergens in Peanut by mutation, recovery mutation and prokaryotic expression. The conformation epitopes of allergens are verified and identified by human serological tests. Finally, animal models were used to validate the identified conformational epitopes, and the specificity of allergen conformational epitopes of different protein families in peanut was analyzed. The role of allergen conformational epitopes of different allergen families in peanut hypersensitivity was clarified.