RNA是生命科学研究的热点，为了更好的研究RNA组成、分布和功能，需要开发更多RNA富集新技术。尤其是备受关注的RNA 颗粒和ncRNA的研究，需要分离富集特定范围内RNA的方法，然而这一方法目前还处于空白。本项目基于化学生物学的思路和手段，通过分子探针的参与，建立特定区域范围内RNA分离、富集及高通量测序方法，对RNA在细胞内空间分布以及RNA在蛋白/核酸相互作用的研究有着十分重要的意义。项目研究内容和思路为，借鉴蛋白质组学中特定区域蛋白质富集方法，通过分子探针对RNA进行修饰，使其模拟蛋白质带上氨基酸残基，因此可以在生物体内被酶催化并发生生物素化得到富集。通过酶与目标蛋白的融合，可以实现活细胞中目标蛋白附近特定区域内的RNA分离和富集，除了本项目所提及生物学问题外，还将对RNA-染色体相互作用、RNA-RNA相互作用等这些新兴领域提供技术基础，有助于实现一些复杂生命活动的深入研究。

The study of RNA has been one of the research focuses in biological science. It is very important to develop more RNA isolation methods to study the constituent, distribution and function. For example, for the research of ncRNA and RNA granule, which is the very hot topic, need the method to isolate RNA within a particular area. However, there is no way for RNA isolation like that. This project wants to develop an in vivo RNA proximity-dependent isolation, enrichment and sequencing method based on chemical probe by chemical biology principle, which is very significant for the study of RNA/protein interactions and RNA distribution inside of cell. This project is inspired by the proximity-dependent techniques for proteomics, and will modify the RNA firstly by chemical probes. This modification will add the function group of amino acid to RNA that make RNA similar with protein, which can be biotinylated by the enzyme used in proximity-dependent techniques for proteomics. Based on the fusion between RNA and protein, this technique can isolate and enrich the RNA around target protein in vivo, which is very useful for the solution of the biological questions in this project. In addition, it is also helpful for the new emerging field of RNA-chromatin interactions and RNA-RNA interactions, which contributes to the intensive study of some complicated biological events.