脂质体作为运载体系是生物医药和食品化工等领域的国际前沿技术和研究热点，但膜结构修饰、药物控释和有效靶向性等问题有待深入。本项目在前期制备层层自组装修饰脂质体有效提高其物化稳定性的基础上，拟采用大尺寸－小尺度－大尺寸制备技术和正负聚电解质静电沉积作用修饰策略制备层层自组装修饰脂质体（LCL），采用现代分析手段表征其结构（光谱结构、微观结构、粒子特性、膜特性），以经典DLVO理论计算其理论稳定性，探讨LCL结构特性和力学行为的变化影响；通过测定其细胞摄取性质（细胞相容性、摄取动力学、途径、定位和内分布等），以耗散动力学模拟LCL内吞过程，探讨LCL结构特性变化与细胞摄取性质的关系；通过测定模拟胃肠道、物理和化学诱导环境及细胞环境下释放的变化，构建动力学释放模型，探讨LCL的结构特性变化与其体内体外释放性质的关系，为深入研究脂质体膜结构的有效调控、细胞靶向性和药物控释行为提供新的思路和理论参考。

Liposome as a deliver system is an nternational advanced technique and research hotspot in biological medicine, foodstuff chemical and other fields. However, there are still issues including membrane structure modification, drug control release, efficient drug targeting greatly needed to be further studied. Thus, in this work, layer-by-layer self-assembly coated liposome (LCL) will be prepared based on our previous work by large scale-small scale-large scale technique together with alternate coating strategy with positive and negative polyelectrolytes, in which physical and chemical stability of liposome have been significantly improved by layer-by-layer self-assembly coating. Structure properties (spectral structures, microstructure, the properties of particles and membrane), and theoretical stability ratio will be analyzed by modern instruments and classical DLVO theory, which aim to discuss variation of structure properties and mechanical behavior. In addition, cellular uptake properties including cellular compatibility, cellular uptake kinetics, cellular mechanism, and cellular intracellular distribution will be determined. Then, dissipative particle dynamics (DPD) method will be introduced to simulate the process of endocytosis of LCL, which aim to establish the relationship between structure properties and cellular uptake properties. Release properties of LCL during in vitro environment (simulated gastrointestinal tract, physical and chemical inducing) and cellular environment will be measured. Then, release model will be further to establish, which plant to illuminate the relationship between structure properties and release characteristic. It aims to provide new idea and theoretic reference for in-depth liposomal researches including effective approaches to adjust membrane structure, drug control release and efficient drug targeting.