自组装形成类似细胞膜的脂质体，作为一种技术前沿的功能成分运载体是生物医药、食品化工等领域的研究热点。课题组前期发现，采用动态高压微射流（DHPM）技术制备各种亲水亲脂性芯材的脂质体结构差异与其理化和功能性质密切相关，但脂质体制备与膜结构的形成机理、结构与热力学稳定性的关系有待深入。为此，本项目采用DHPM驱动荧光标记空白脂质体二次组装成纳米脂质体，借助激光散射等技术测定DHPM处理前后脂质体的物化特性(粒径与分布、表面电荷、相变温度、表面张力和相对浊度)的影响；采用现代显微镜等技术表征脂质体膜的结构特性(层数、间距、厚度、完整性和通透性等)的变化，构建DHPM驱动脂质体二次组装的动力学模型并阐析其机理；研究脂质体的热力学(物理、化学和长期)稳定性变化，结合结构特性差异进行多因素相关性分析，揭示脂质体结构与热力学稳定性的作用关系，为脂质体二次组装理论和结构与性质有效调控等研究提供理论借鉴。

As a functional components delivery system, liposomes, which are self-assembled and cell-resembled, have been remarkable developed in pharmaceutical biology and food chemistry in recent years. In our previous studies,liposomes encapsulated different hydrophilic and hydrophobic contents were prepared by dynamic high pressure microfluidization (DHPM). The liposomal structures play important role in their physicalchemical properties and functinal properties. However, the relationships between preparation method and the formation mechanism of membrane, and liposomal structures and thermodynamic stability need to be further studied. Thus, the present study focuses on fluorescence labeled blank nanoliposomes formed by second-assembling forced by DHPM. The physicochemical properties of liposomes, including diameter, size distribution, zeta potential, phase-transition temperature, surface tension and relative turbidity, are measured by high-tech approaches such as laser scattering technique. The structure, distance, thickness, integrity and permeability of both knid of liposomal membrane are measured according to morden technology such as electron microscope. The second-assembling model of liposomes forced by DHPM could be established and the mechanism would be clarified, according to the changes of liposomal properties and membrane structures. Besides, the thermodynamic stability such as physical, chemical as well as long-term stability of liposomes before or after treated with DHPM is studied. Based on the multi-factors correlation analysis, the relationship between membrane structure and thermodynamic stability would be revealed. Our study may provide some useful technical guidance for the formation mechanism of liposomal second-assembling and quality control.