CRF营养不良发生率高，与CRF预后密切相关，发生机制尚未阐明。研究发现Wnt7a信号通路有调控MSC的功能，促进骨骼肌组织生长和修复，但在CRF营养不良所致骨骼肌萎缩发生中的作用机制尚不清楚。我们通过预实验发现，CRF营养不良动物模型骨骼肌组织Wnt7a表达下调，与骨骼肌萎缩相关。为了深入研究Wnt7a信号通路在CRF营养不良发生中的作用，我们拟通过建立CRF营养不良动物模型和siRNA沉默Wnt7a基因细胞模型，研究Wnt7a-PCP（Fzd7 、Vangl2、Rac、ROCK-2、Rho、JNK）信号通路和Wnt7a-AKT/mTOR（Fzd7、PI3K、Akt、mTOR）信号通路对肌卫星细胞（MSC）功能和活性的调节作用及MSC对骨骼肌萎缩的作用，通过应用肾衰营养胶囊进行干预，探讨Wnt7a调控信号通路在CRF营养不良发生中的作用及肾衰营养胶囊改善CRF营养不良的分子机制。

The incidence of malnutrition with CRF is higher,and closely related to the prognosis of CRF,and the pathogenesis has not been elucidated. Study finds that Wnt7a pathway regulates the function of MSC, and promotes the growth and restoration of skeletal muscle,but the mechanism on skeletal muscle atrophy in CRF with malnutrition is unclear. We found that the expression of Wnt7a in skeletal muscle tissue of animal models of CRF with malnutrition was downregulated,and associated with skeletal muscle atrophy. To profoundly study the effect of Wnt7a pathway on the pathogenesis of malnutrition in CRF, we intend to establish animal model of CRF with malnutrition and siRNA knocking-down of wnt7a gene, to study the role of Wnt7a-PCP（Fzd7 、Vangl2、Rac、ROCK-2、Rho、JNK）pathways and Wnt7a-AKT/mTOR（Fzd7、PI3K、Akt、mTOR）on the function and activity of MSC ,and the effect of MSC in the mechanism of skeletal muscle atrophy, and application of Shenshuai Yingyang capsules, to explore the role of Wnt7a in the pathogenesis of in CRF with malnutrition and the molecular mechanism of Shenshuai Yingyang capsules for treatment of CRF with malnutrition.