凡纳滨对虾抗副溶血弧菌性状的遗传基础及调控机制研究

病害问题是影响对虾产业可持续发展的主要瓶颈，从对虾种质入手进行抗病良种的培育是解决病害问题的重要途径。由于抗病性状表型较难度量，对抗病性状进行遗传估计准确率较低，因此利用传统选育技术培育抗病品种进展缓慢。利用分子育种技术可以加快抗病育种进程，对抗病性状的遗传解析并研究其调控机制是开展分子育种的基础。本项目拟在凡纳滨对虾全基因组序列组装完成的基础上，针对严重危害对虾养殖业的急性肝胰腺坏死综合症的主要病原菌—副溶血弧菌，利用全基因组关联分析筛选对虾抗副溶血弧菌相关分子标记，整合多组学分析定位对虾抗性关键基因，揭示关键基因的功能并阐明其调控对虾抗弧菌性状的分子机制。相关研究成果不但可以丰富和完善甲壳动物免疫学的基础理论，也可为对虾以及其它经济甲壳动物的抗性育种提供重要指导，进一步推动甲壳动物抗性分子育种的实践和应用。

Shrimp disease has been becoming the impediment for the sustainable development of shrimp aquaculture industry. Breeding the disease resistance variety is the fundamental approach to solve the problem of disease. However, progress in the breeding of disease resistance trait in shrimp through classical breeding approaches goes very slowly due to the difficulties including hard phenotypic measurement and low accuracy of estimations on genetic parameters etc. Molecular breeding is very helpful to speed up the breeding process of disease resistance trait, while genetic analyses on the disease resistance traits are the basis. In this project, we will focus on the genetic analysis of shrimp resistance to acute hepatopancreatic necrosis syndrome(AHPNS), which was mainly caused by Vibrio parahemolyticus infection, based on previous progresses in immunity and genetic breeding of penaeid shrimp. With the successful assembly of the whole-genome sequence of Litopeneaus vannamei by our team, molecular markers associated with the resistance to V. parahemolyticus will be screened by GWAS, and the key genes responsible for V. parahemolyticus resistance will be fine mapped in the shrimp genome by integration of Multi-omics data. The function of these key genes will be studied, and the molecular regulation mechanism of them will be clarified. The predicted achievements in the project will not only enrich the theoretical knowledge of crustacean immunity, but also provide guidance for disease resistance breeding in shrimp and other crustacean species, and further speed up the application of molecular breeding for disease resistance in crustaceans.