A human-curated annotation of the Candida albicans genome.
A human-curated annotation of the Candida albicans genome.
复制标题
白色念珠菌基因组的人类策划注释。
DOI：
10.1371/journal.pgen.0010001
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发表时间：
2005-07
期刊：
影响因子：
4.5
通讯作者：
Nantel, A
中科院分区：
文献类型：
--
作者：
Braun, BR;Hoog, MV;d'Enfert, C;Martchenko, M;Dungan, J;Kuo, A;Inglis, DO;Uhl, MA;Hogues, H;Berriman, M;Lorenz, M;Levitin, A;Oberholzer, U;Bachewich, C;Harcus, D;Marcil, A;Dignard, D;Iouk, T;Zito, R;Frangeul, L;Tekaia, F;Rutherford, K;Wang, E;Munro, CA;Bates, S;Gow, NA;Hoyer, LL;Köhler, G;Morschhäuser, J;Newport, G;Znaidi, S;Raymond, M;Turcotte, B;Sherlock, G;Costanzo, M;Ihmels, J;Berman, J;Sanglard, D;Agabian, N;Mitchell, AP;Johnson, AD;Whiteway, M;Nantel, A
真菌病原体白色念珠菌的基因组的最新测序和组装使用简单的自动化程序来鉴定推定的基因。我们通过手工和额外的生物信息学资源对整个组装进行了审查，以准确地绘制和描述6，354个基因，并确定了246个基因，其原始数据库条目包含影响其阅读框架的测序错误（或可能的突变）。与其他真菌基因组的比较允许鉴定许多真菌特异性基因，这些基因可能是抗真菌治疗的目标。我们还观察到，与其他真菌相比，C。白色念珠菌基因组特别富含短序列重复。最后，我们改进的注释允许对几个多基因家族进行详细的分析，比较基因组研究表明，C。白色念珠菌具有大得多的分解代谢范围，编码呼吸复合物1、几种新的氧化还原酶和酮体降解酶、丙二酰-CoA和烯酰-CoA载体、几种新的氨基酸降解酶、多种分泌的分解代谢脂肪酶和蛋白酶以及大量的转运蛋白以同化所得的营养物。这些努力的结果将确保念珠菌研究界拥有统一且全面的基因组信息，用于医学研究以及未来的诊断和治疗应用。 白色念珠菌是一种常见的真菌病原体，通常引起浅表感染（鹅口疮和阴道炎）。然而，估计有30%的严重真菌感染，大多数是由于念珠菌，导致死亡。风险最大的人包括器官移植后服用免疫抑制药物的人，艾滋病毒感染者，早产儿和接受化疗的癌症患者。目前针对这种病原体的治疗方法由于抗真菌药物的显着次要作用而变得更加困难，这些药物靶向的蛋白质也在人类宿主中发现。真菌病原体C.白色念珠菌使用简单的自动化程序来鉴定推定的基因。在这里，我们报告了这种生物体基因组序列中存在的6，354个基因的详细注释，基本上是在编写C.白色念珠菌基因组。与其他真菌基因组的比较允许鉴定许多真菌特异性基因，这些基因在人类基因组中不存在，其产物可能是抗真菌治疗的目标。因此，这些努力的结果将确保念珠菌研究界拥有统一和全面的基因组信息，用于医学研究，开发功能性基因组工具以及未来的诊断和治疗应用。
Recent sequencing and assembly of the genome for the fungal pathogen Candida albicans used simple automated procedures for the identification of putative genes. We have reviewed the entire assembly, both by hand and with additional bioinformatic resources, to accurately map and describe 6,354 genes and to identify 246 genes whose original database entries contained sequencing errors (or possibly mutations) that affect their reading frame. Comparison with other fungal genomes permitted the identification of numerous fungus-specific genes that might be targeted for antifungal therapy. We also observed that, compared to other fungi, the protein-coding sequences in the C. albicans genome are especially rich in short sequence repeats. Finally, our improved annotation permitted a detailed analysis of several multigene families, and comparative genomic studies showed that C. albicans has a far greater catabolic range, encoding respiratory Complex 1, several novel oxidoreductases and ketone body degrading enzymes, malonyl-CoA and enoyl-CoA carriers, several novel amino acid degrading enzymes, a variety of secreted catabolic lipases and proteases, and numerous transporters to assimilate the resulting nutrients. The results of these efforts will ensure that the Candida research community has uniform and comprehensive genomic information for medical research as well as for future diagnostic and therapeutic applications. Candida albicans is a commonly encountered fungal pathogen usually responsible for superficial infections (thrush and vaginitis). However, an estimated 30% of severe fungal infections, most due to Candida, result in death. Those who are most at risk include individuals taking immune-suppressive drugs following organ transplantation, people with HIV infection, premature infants, and cancer patients undergoing chemotherapy. Current therapies for this pathogen are made more difficult by the significant secondary effects of anti-fungal drugs that target proteins that are also found in the human host. Recent sequencing and assembly of the genome for the fungal pathogen C. albicans used simple automated procedures for the identification of putative genes. Here, we report a detailed annotation of the 6,354 genes that are present in the genome sequence of this organism, essentially writing the dictionary of the C. albicans genome. Comparison with other fungal genomes permitted the identification of numerous fungus-specific genes that are absent from the human genome and whose products might be targeted for antifungal therapy. The results of these efforts will thus ensure that the Candida research community has uniform and comprehensive genomic information for medical research, for the development of functional genomic tools as well as for future diagnostic and therapeutic applications.
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