Coriandrum sativum attenuates microglia mediated neuroinflammation and MPTP-induced behavioral and oxidative changes in Parkinson's disease mouse model.
Coriandrum sativum attenuates microglia mediated neuroinflammation and MPTP-induced behavioral and oxidative changes in Parkinson's disease mouse model.
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DOI：
10.17179/excli2021-3668
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发表时间：
2021
期刊：
影响因子：
4.6
通讯作者：
Kopalli SR
中科院分区：
文献类型：
--
作者：
Koppula S;Alluri R;Kopalli SR
芫荽（科：伞形科; C. sativum）是一种广泛用作香料和传统药物的潜在草药。本文研究了C.研究了大蒜果实提取物（CSE）对脂多糖（LPS）刺激的BV-2小胶质细胞介导的体外神经炎症和1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）诱导的帕金森病（PD）动物模型的体内保护作用。CSE（25、50和100 μg/mL）可显著抑制LPS刺激的BV-2小胶质细胞中一氧化氮（NO）、诱导型NO合酶、环氧合酶-2、白细胞介素-6和肿瘤坏死因子-α的升高（p < 0.05 ~ p < 0.001）。此外，CSE抑制BV-2小胶质细胞中LPS诱导的核因子κ-β活化和IκB-α磷酸化。在体内研究中，CSE（100、200和300 mg/kg）改善MPTP（25 mg/kg，i. p.）转棒、被动回避和旷场实验结果显示，大鼠的运动、认知和行为功能均发生显著性变化（P < 0.05 ~ P < 0.001）。MPTP诱导的脑氧化酶水平变化，如超氧化物歧化酶、过氧化氢酶和脂质过氧化反应，在200和300 mg/kg CSE处理后显著恢复（分别为p < 0.01和p < 0.001）。CSE的高效薄层色谱指纹图谱分析显示，槲皮素和山奈酚-3O-葡萄糖苷作为可识别的化合物的几个独特的峰。总之，我们的研究表明，CSE在体外减弱LPS刺激的小胶质细胞中的神经炎症过程，并在体内恢复MPTP诱导的行为缺陷和脑氧化酶状态，证明其在治疗PD中观察到的神经炎症和氧化应激介导的神经变性中的治疗潜力。
Coriandrum sativum Linn. (family: Umbelliferae; C. sativum), is a potential herb widely used as a spice and traditional medicine. In the present work, the effects of C. sativum fruit extract (CSE), against lipopolysaccharide (LPS)-stimulated BV-2 microglia-mediated neuroinflammation in vitro and 1-methyl-4 phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) animal model in vivo was investigated. LPS-stimulated increase in nitric oxide (NO), inducible NO synthase, cyclooxygenase-2, interleukin-6 and tumor necrosis factor-alpha were significantly (p < 0.05 ~ p < 0.001) inhibited by CSE (25, 50 and 100 μg/mL) in BV-2 microglial cells. Further, CSE inhibited the LPS-induced nuclear factor of kappa-beta activation and IκB-α phosphorylation in BV-2 microglia. In vivo studies, CSE (100, 200 and 300 mg/kg) ameliorated the MPTP (25 mg/kg, i.p.)-induced changes in locomotor, cognitive and behavior functions evaluated by rotarod, passive avoidance and open field test significantly (p < 0.05 ~ p < 0.001). The MPTP-induced changes in brain oxidative enzyme levels such as superoxide dismutase, catalase, and lipid peroxidation were significantly (p < 0.01 and p < 0.001 at 200 and 300 mg/kg, respectively) restored with CSE treatment. High-performance thin-layer chromatography fingerprinting analysis of CSE exhibited several distinctive peaks with quercetin and kaempferol-3O-glucoside as identifiable compounds. In conclusion, our study indicated that CSE attenuated neuroinflammatory processes in LPS-stimulated microglia in vitro and restored the MPTP-induced behavioral deficits and brain oxidative enzyme status in vivo proving its therapeutic potential in the treatment of neuroinflammatory and oxidative stress-mediated neurodegeneration seen in PD.
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影响因子：
14.8
作者：
Jackson-Lewis, Vernice;Przedborski, Serge
通讯作者：
Przedborski, Serge
DOI：
10.1016/j.bjp.2014.07.018
发表时间：
2014-08-01
期刊：
Revista Brasileira de Farmacognosia
影响因子：
--
作者：
Cortés, Natalie;Mora, Cristina;Osorio, Edison
通讯作者：
Osorio, Edison
影响因子：
2.9
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
通讯作者：
TANNENBAUM, SR
影响因子：
5.4
作者：
Jabeen, Qaiser;Bashir, Samra;Gilani, Anwar H.
通讯作者：
Gilani, Anwar H.
天然产物及其化学衍生物介导的神经保护
影响因子：
6.1
作者：
Fei F;Su N;Li X;Fei Z
通讯作者：
Fei Z