The expression of one ankyrin pk2 allele of the WO prophage is correlated with the Wolbachia feminizing effect in isopods.
The expression of one ankyrin pk2 allele of the WO prophage is correlated with the Wolbachia feminizing effect in isopods.
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DOI：
10.1186/1471-2180-12-55
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发表时间：
2012-04-12
期刊：
影响因子：
4.2
通讯作者：
Grève P
中科院分区：
文献类型：
--
作者：
Pichon S;Bouchon D;Liu C;Chen L;Garrett RA;Grève P
母系遗传的沃尔巴克氏变形杆菌(α)是线虫和节肢动物的专性内共生体，引起多种生殖变化，包括细胞质不亲和性(CI)和雌性化。由等足目普通Armadillidium umgare携带的雌性wVulC Wolbchia菌株的基因组已经测序，现在处于最后组装步骤。它含有异常多的含有锚定基序的基因，其中两个与噬菌体相关的pk1和pk2基因同源，被认为是库蚊CI表型的贡献者。这些基因编码可能的细菌效应器，通过其锚定基序介导沃尔巴克氏菌-宿主蛋白-蛋白质相互作用。为了测试这些Wolbachia同源基因是否可能参与改变陆地等足类动物的繁殖，我们测定了pk1和pk2基因在3个诱导CI的Wolbachia菌株和5个诱导其等足目宿主雌性化的Wolbachia菌株中的分布和表达。除了高度保守的基因外，我们还发现菌株之间存在显著的拷贝数差异，这与原噬菌体多样性有关。转录分析显示，只有一个pk2等位基因(Pk2b2)在等足类雌性化沃尔巴克氏菌株中表达。这些结果表明，有必要研究Wolbachia ankyrin基因产物，特别是Pk2基因产物的功能，以及它们的宿主靶标在宿主性别操纵方面的作用。
The maternally inherited α-Proteobacteria Wolbachia pipientis is an obligate endosymbiont of nematodes and arthropods, in which they induce a variety of reproductive alterations, including Cytoplasmic Incompatibility (CI) and feminization. The genome of the feminizing wVulC Wolbachia strain harboured by the isopod Armadillidium vulgare has been sequenced and is now at the final assembly step. It contains an unusually high number of ankyrin motif-containing genes, two of which are homologous to the phage-related pk1 and pk2 genes thought to contribute to the CI phenotype in Culex pipiens. These genes encode putative bacterial effectors mediating Wolbachia-host protein-protein interactions via their ankyrin motifs. To test whether these Wolbachia homologs are potentially involved in altering terrestrial isopod reproduction, we determined the distribution and expression of both pk1 and pk2 genes in the 3 Wolbachia strains that induce CI and in 5 inducing feminization of their isopod hosts. Aside from the genes being highly conserved, we found a substantial copy number variation among strains, and that is linked to prophage diversity. Transcriptional analyses revealed expression of one pk2 allele (pk2b2) only in the feminizing Wolbachia strains of isopods. These results reveal the need to investigate the functions of Wolbachia ankyrin gene products, in particular those of Pk2, and their host targets with respect to host sex manipulation.
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古老的内共生体内插入序列的强烈替代活性。
影响因子：
10.7
作者：
Cordaux, Richard;Pichon, Samuel;Ling, Alison;Perez, Philippe;Delaunay, Carine;Vavre, Fabrice;Bouchon, Didier;Greve, Pierre
通讯作者：
Greve, Pierre
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
4.7
作者：
Bouchon, D;Rigaud, T;Juchault, P
通讯作者：
Juchault, P
影响因子：
10.7
作者：
Gavotte, Laurent;Henri, Helene;Vavre, Fabrice
通讯作者：
Vavre, Fabrice
DOI：
10.1002/prot.340170405
发表时间：
1993-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
BORK, P
通讯作者：
BORK, P
影响因子：
9.6
作者：
Fenn, K;Blaxter, M
通讯作者：
Blaxter, M