Genetic, biological and epidemiological study on a cluster of H9N2 avian influenza virus infections among chickens, a pet cat, and humans at a backyard farm in Guangxi, China.
Genetic, biological and epidemiological study on a cluster of H9N2 avian influenza virus infections among chickens, a pet cat, and humans at a backyard farm in Guangxi, China.
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中国广西某后院农场鸡、宠物猫和人类发生的 H9N2 禽流感病毒聚集性感染的遗传、生物学和流行病学研究
DOI：
10.1080/22221751.2022.2143282
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发表时间：
2023-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在2018年10月的一次调查中，广西两名以腹泻、轻度腹痛和轻度关节痛症状为特征的人中国被确诊为H9N2型禽流感病毒感染者。从两名患者之一、一只宠物猫和一只死鸡(分别从患者的肺和肾组织中分离出两株)中分离到四株H9N2AIV，这些鸡是从后院养殖场饲养的。流行病学调查表明，新买的鸡首先死亡，随后两名主人和一只猫出现临床症状。此外，这两个个体具有较高的H9N2特异性血凝抑制和微中和抗体。在4株H9N2分离株中，所有基因的核苷酸序列同源性为99.9%~100%，血凝素(HA)T138A位于受体结合域(RBD)上，这是由女性患者分离株特有的核苷酸多态引起的。此外，在这三种寄主的分离物中都发现了RBD上的HA K137N。重要的是，这四个H9N2分离株对人型受体(α2-6-SA)表现出唯一的结合偏好，并能在小鼠身上复制并引起病理变化。系统发育分析表明，这4个分离物聚在一起，属于C1.2分支，Y280谱系。此外，人类来源的H9N2病毒在基因上是不同的，并与广泛存在的家禽来源的H9N2AIV病毒穿插在一起。这些结果表明，H9N2AIV在公共卫生中具有很高的风险，为了动物和人类的健康，应该考虑和采取有效的预防和控制措施。
ABSTRACT During an investigation in October 2018, two people with diarrhoea, mild abdominal pain, and mild arthralgia symptoms in Guangxi, China, were identified as infected by H9N2 avian influenza virus (AIV). Four H9N2 AIVs were isolated from one of two patients, a pet cat, and a dead chicken (two respective isolates from its lung and kidney tissues) bred by the patients at a backyard farm. Epidemiological investigation indicated that the newly bought chicken died first, and clinical syndromes appeared subsequently in the two owners and one cat. Furthermore, the two individuals possessed high H9N2-specific hemagglutination inhibition and microneutralization antibodies. Shared nucleotide sequence identity (99.9% – 100%) for all genes was detected in the four H9N2 isolates, and hemagglutinin (HA) T138A located on the receptor binding domain (RBD), resulted from nucleotide polymorphisms that were exclusively found in the isolate from the female patient. Moreover, HA K137N on the RBD was found in isolates from these three host species. Importantly, these four H9N2 isolates presented an exclusive binding preference for the human-type receptor (α2-6-SA), and could replicate and cause pathological changes in mice. Phylogenetic analyses showed that these four isolates clustered together and belonged to clade C1.2, lineage Y280. In addition, H9N2 viruses of human origin are genetically divergent and interspersed with the widespread poultry-origin H9N2 AIVs. All these results indicate a high risk of H9N2 AIVs in public health, and effective prevention and control measures against H9N2 AIVs should be considered and performed for both animal and human health.
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DOI：
10.1084/jem.78.2.99
发表时间：
1943-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hirst GK
通讯作者：
Hirst GK
影响因子：
9.4
作者：
Butt, KM;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
影响因子：
6.7
作者：
Li X;Shi J;Guo J;Deng G;Zhang Q;Wang J;He X;Wang K;Chen J;Li Y;Fan J;Kong H;Gu C;Guan Y;Suzuki Y;Kawaoka Y;Liu L;Jiang Y;Tian G;Li Y;Bu Z;Chen H
通讯作者：
Chen H
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM
影响因子：
4.4
作者：
Ma, Chunna;Cui, Shujuan;Wang, Quanyi
通讯作者：
Wang, Quanyi