Separation methods used for Scutellaria baicalensis active components.
Separation methods used for Scutellaria baicalensis active components.
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DOI：
10.1016/j.jchromb.2004.06.045
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发表时间：
2004-12-05
期刊：
影响因子：
--
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Li HB;Jiang Y;Chen F
黄芩是我国应用最广泛的中草药之一。其根被用于抗炎、抗癌、抗病毒和抗细菌感染呼吸道和胃肠道，清热、润燥、泻火、解毒、降低总胆固醇水平和降低血压。其主要活性成分为黄芩苷、黄芩素、汉黄素和苦木素A。本文综述了用于这些活性成分的定量和定性测定的各种分离、检测和鉴定技术。介绍了高效液相色谱、高速逆流色谱、薄层色谱、毛细管电泳法和胶束电动毛细管色谱在这些活性成分的分离和测定中的应用。还给出了用高效液相色谱-质谱法和气相色谱-质谱法鉴定复杂基质中这些活性成分及其代谢物的实例。对这些分离鉴定方法的优点和局限性进行了评价和讨论。
Scutellaria baicalensis Georgi is one of the most widely used traditional Chinese herbal medicines. Its roots have been used for anti-inflammation, anticancer, antiviral and antibacterial infections of the respiratory and the gastrointestinal tract, cleaning away heat, moistening aridity, purging fire, detoxifying toxicosis, reducing the total cholesterol level and decreasing blood pressures. Baicalin, baicalein, wogonin and oroxylin A are its main active components. This review provides an overview of various separation, detection, and identification techniques employed for the quantitative and qualitative determination of these active components. Applications of high-performance liquid chromatography, high-speed counter-current chromatography, thin layer chromatography, capillary electrophoresis, and micellar electrokinetic capillary chromatography to the separation and determination of these active components are described. Examples of identification of these active components and their metabolites in complex matrices by high-performance liquid chromatography–mass spectrometry and gas chromatography–mass spectrometry are also presented. The advantages and limitations of these separation and identification methods are assessed and discussed.
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DOI：
10.1254/jjp.27.31
发表时间：
1977-01-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
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通讯作者：
SAKAMOTO, K
影响因子：
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Táborská, E
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DOI：
10.1016/s1570-0232(02)00288-x
发表时间：
2002-08-05
影响因子：
3
作者：
Chen, XY;Wang, HY;Zhong, DF
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