Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold.
Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold.
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DOI：
10.1016/j.biomaterials.2010.10.056
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发表时间：
2011-02
期刊：
影响因子：
14
通讯作者：
Jun, Ho-Wook
中科院分区：
文献类型：
--
作者：
Blakeney, Bryan A.;Tambralli, Ajay;Anderson, Joel M.;Andukuri, Adinarayana;Lim, Dong-Jin;Dean, Derrick R.;Jun, Ho-Wook
传统静电纺丝的一个限制因素是，静电纺丝支架完全由紧密堆积的纤维层组成，由于片状组装过程，这些纤维层仅提供表面多孔结构。这种不可避免的特性阻碍了整个纳米纤维支架的细胞浸润和生长。已经尝试了许多策略来克服这一挑战，包括掺入纳米颗粒，使用更大的微纤维，或去除嵌入的盐或水溶性纤维以增加孔隙率。然而，这些方法仍然产生片状纳米纤维支架，未能产生具有良好结构完整性的多孔三维支架。因此，我们开发了一种三维棉球状静电纺丝支架，其由低密度和未压缩方式的纳米纤维积累组成。代替传统的平板收集器，使用接地球形盘和针状探针阵列来创建聚焦、低密度、未压缩的纳米纤维（FLUF）网状支架。扫描电子显微镜显示，与传统的静电纺丝支架相比，棉球状支架由直径相似但孔更大且结构更不致密的静电纺丝纳米纤维组成。此外，激光共聚焦显微镜显示在整个棉花球状支架的深度上具有开放的多孔性和松散的堆积结构，与传统电纺支架的表面多孔和紧密堆积结构形成对比。接种在棉花球样支架上的细胞在生长7天后渗透到支架中，而传统的静电纺丝支架没有渗透生长。定量分析显示，在7天的时间内，细胞在棉球状支架上的生长速率高出约40%，这可能是由于三维支架内的向内生长空间增加。总的来说，这种方法组装了一种纳米纤维支架，这种支架对于高度多孔的互连性更有利，并且展示了应对电纺支架当前挑战的巨大潜力。
A limiting factor of traditional electrospinning is that the electrospun scaffolds consist entirely of tightly packed nanofiber layers that only provide a superficial porous structure due to the sheet-like assembly process. This unavoidable characteristic hinders cell infiltration and growth throughout the nanofibrous scaffolds. Numerous strategies have been tried to overcome this challenge, including the incorporation of nanoparticles, using larger microfibers, or removing embedded salt or water-soluble fibers to increase porosity. However, these methods still produce sheet-like nanofibrous scaffolds, failing to create a porous three-dimensional scaffold with good structural integrity. Thus, we have developed a three-dimensional cotton ball-like electrospun scaffold that consists of an accumulation of nanofibers in a low density and uncompressed manner. Instead of a traditional flat-plate collector, a grounded spherical dish and an array of needle-like probes were used to create a Focused, Low density, Uncompressed nanoFiber (FLUF) mesh scaffold. Scanning electron microscopy showed that the cotton ball-like scaffold consisted of electrospun nanofibers with a similar diameter but larger pores and less dense structure compared to the traditional electrospun scaffolds. In addition, laser confocal microscopy demonstrated an open porosity and loosely packed structure throughout the depth of the cotton ball-like scaffold, contrasting the superficially porous and tightly packed structure of the traditional electrospun scaffold. Cells seeded on the cotton ball-like scaffold infiltrated into the scaffold after 7 days of growth, compared to no penetrating growth for the traditional electrospun scaffold. Quantitative analysis showed approximately a 40% higher growth rate for cells on the cotton ball-like scaffold over a 7 day period, possibly due to the increased space for in-growth within the three-dimensional scaffolds. Overall, this method assembles a nanofibrous scaffold that is more advantageous for highly porous interconnectivity and demonstrates great potential for tackling current challenges of electrospun scaffolds.
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作者：
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用于皮肤组织工程的电纺聚多（乳酸 - 乙醇酸）支架。
影响因子：
14
作者：
Kumbar, Sangamesh G.;Nukavarapu, Syam P.;James, Roshan;Nair, Lakshmi S.;Laurencin, Cato T.
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Laurencin, Cato T.